Determination of amlodipine besylate by adsorptive square-wave anodic stripping voltammetry on glassy carbon electrode in tablets and biological fluids

The adsorptive and electrochemical behavior of amlodipine besylate on a glassy carbon electrode were explored in Britton-Robinson buffer solution by using cyclic and square-wave voltammetry. Cyclic voltammetric studies indicated the oxidation of amlodipine besylate at the electrode surface through a single two-electron irreversible step and fundamentally controlled by adsorption. The solution conditions and instrumental parameters were optimized for the determination of the authentic drug by adsorptive square-wave stripping voltammetry. Amlodipine besylate gave a sensitive adsorptive oxidation peak at 0.510 V (versus Ag/AgCl). The oxidation peak was used to determine amlodipine besylate in range 4.0 x 10(-8) to 2.0 x 10(-6) with a detection limit of 1.4 x 10(-8) M. The procedure was successfully applied for the assay of amlodipine besylate in tablets (Norvasc)(R). The percentage recoveries were in agreement with those obtained by the reference method. Applicability to assay the drug in urine and serum samples was illustrated. The mean percentage recoveries were 96.31 +/- 1.18 and 96.98 +/- 1.17, respectively. The proposd method used for monotoring clinically relevant concntrations of drug in human urine and serum. (C) 2003 Elsevier B.V. All rights reserved.