Surface-attached molecular beacons light the way for DNA sequencing

被引:40
作者
Palecek, E [1 ]
机构
[1] Acad Sci Czech Republ, Inst Biophys, CS-61265 Brno, Czech Republic
关键词
D O I
10.1016/j.tibtech.2003.11.009
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Rapid testing of DNA and RNA nucleotide sequences is required for various research protocols including wide-scale genetic testing, diagnostics, fast detection of biological warfare agents, environmental testing and forensic medicine. At present many laboratories are interested in research and development of an inexpensive, easy-to-use, fast-response device for this purpose. Various methods based on acoustic, electronic and optical detection of the DNA hybridization event have been reported.
引用
收藏
页码:55 / 58
页数:4
相关论文
共 20 条
[1]   Capillary gel electrophoresis with sinusoidal voltammetric detection: A strategy to allow four-"color" DNA sequencing [J].
Brazill, SA ;
Kim, PH ;
Kuhr, WG .
ANALYTICAL CHEMISTRY, 2001, 73 (20) :4882-4890
[2]   Stem-loop oligonucleotides: a robust tool for molecular biology and biotechnology [J].
Broude, NE .
TRENDS IN BIOTECHNOLOGY, 2002, 20 (06) :249-256
[3]  
DRUMMOND GT, 1999, NAT BIOTECHNOL, V21, P1192
[4]   Hybridization-based unquenching of DNA hairpins on Au surfaces: Prototypical "molecular beacon" biosensors [J].
Du, H ;
Disney, MD ;
Miller, BL ;
Krauss, TD .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (14) :4012-4013
[5]   Single-mismatch detection using gold-quenched fluorescent oligonucleotides [J].
Dubertret, B ;
Calame, M ;
Libchaber, AJ .
NATURE BIOTECHNOLOGY, 2001, 19 (04) :365-370
[6]   Electrochemical interrogation of conformational changes as a reagentless method for the sequence-specific detection of DNA [J].
Fan, CH ;
Plaxco, KW ;
Heeger, AJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (16) :9134-9137
[7]   Label-free determination of picogram quantities of DNA by stripping voltammetry with solid copper amalgam or mercury electrodes in the presence of copper [J].
Jelen, F ;
Yosypchuk, B ;
Kourilová, A ;
Novotny, L ;
Palecek, E .
ANALYTICAL CHEMISTRY, 2002, 74 (18) :4788-4793
[8]  
MARMUR J, 1963, PROG NUCLEIC ACID RE, V1, P231
[9]   OSCILLOGRAPHIC POLAROGRAPHY OF HIGHLY POLYMERIZED DEOXYRIBONUCLEIC ACID [J].
PALECEK, E .
NATURE, 1960, 188 (4751) :656-657
[10]   New approaches in the development of DNA sensors: hybridization and electrochemical detection of DNA and RNA at two different surfaces [J].
Palecek, E ;
Fojta, M ;
Jelen, F .
BIOELECTROCHEMISTRY, 2002, 56 (1-2) :85-90