Carbamylated erythropoietin improves angiogenesis and protects the kidneys from ischemia-reperfusion injury

被引：38

作者：

Imamura, Ryoichi ^{[2
]}

Okumi, Masayoshi ^{[2
]}

Isaka, Yoshitaka ^{[1
,4
]}

Ichimaru, Naotsugu ^{[2
]}

Moriyarna, Toshiki ^{[3
,4
]}

Imai, Enyu ^{[4
]}

Nonomura, Norio ^{[2
]}

Takahara, Shiro ^{[1
]}

Okuyama, Akihiko ^{[2
]}

机构：

[1] Osaka Univ, Grad Sch Med, Dept Adv Technol & Transplantat, Suita, Osaka 5650871, Japan

[2] Osaka Univ, Dept Urol, Grad Sch Med, Suita, Osaka 5650871, Japan

[3] Osaka Univ, Hlth Care Ctr, Osaka 5600043, Japan

[4] Osaka Univ, Grad Sch Med, Dept Nephrol, Suita, Osaka 5650871, Japan

来源：

CELL TRANSPLANTATION | 2008年 / 17卷 / 1-2期

关键词：

angiogenesis; carbamylated erythropoietin; ischemia-reperfusion injury; kidney; apoptosis;

D O I：

10.3727/000000008783907044

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Several studies have shown that erythropoietin (EPO) can protect the kidneys from ischemia-reperfusion injury and can raise the hemoglobin (Hb) concentration. Recently, the EPO molecule modified by carbamylation (CEPO) has been identified and was demonstrated to be able to protect several organs without increasing the Hb concentration. We hypothesized that treatment with CEPO would protect the kidneys, partly due to the increased peritubular capillaries. The therapeutic effect of CEPO was evaluated using an endothelial tube formation assay in vitro, and a rat ischemia-reperfusion injury model in vivo. EPO treatment showed the tendency of increased tube formation, while CEPO treatment induced more capillary-like formation than EPO. Ischemia-reperfusion-induced kidneys exhibited characteristic nuclei of apoptosis in tubular epithelial cells with decreased peritubular capillaries, while EPO treatment inhibited tubular apoptosis with preserved endothelial cells. Moreover, CEPO-treated kidneys showed minimal tubular apoptosis with increased peritubular capillary endothelial cells. In conclusion, we identified a new therapeutic approach using CEPO to protect kidneys from ischemia-reperfusion injury by promoting angiogenesis.

引用

页码：135 / 141

页数：7

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