The comet assay for the evaluation of the genetic hazard of pollutants in cetaceans: Preliminary results on the genotoxic effects of methyl-mercury on the bottle-nosed dolphin (Tursiops truncatus) lymphocytes in vitro

被引：56

作者：

Betti, C

Nigro, M

机构：

[1] UNIV PISA, DIPARTIMENTO BIOMED SPERIMENTALE, SEZ BIOL & GENET, I-56126 PISA, ITALY

[2] UNIV PISA, DIPARTIMENTO SCI AMBIENTE & TERR, I-56100 PISA, ITALY

来源：

MARINE POLLUTION BULLETIN | 1996年 / 32卷 / 07期

关键词：

D O I：

10.1016/0025-326X(96)84573-5

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

In this study the single cell microgel electrophoresis (Comet assay) was used to evaluate the genetic effects of methyl-mercury in dolphin (Tursiops truncatns) lymphocytes in the dose range 1-8 mu g ml(-1). The results of in vitro exposure showed that methyl-Hg induces DNA single-strand breaks and cytotoxicity in a dose-dependent manner. Dolphin lymphocytes have greater resistance to the genotoxic and cytotoxic effects of methyl-Hg than human and rat cells. The ecological significance of these results are discussed. Copyright (C) 1996 Elsevier Science Ltd

引用

页码：545 / 548

页数：4

共 24 条

[1] COMPARATIVE-STUDY OF MERCURY ACCUMULATION IN DOLPHINS (STENELLA-COERULEOALBA) FROM FRENCH ATLANTIC AND MEDITERRANEAN COASTS
ANDRE, J
BOUDOU, A
RIBEYRE, F
BERNHARD, M
[J]. SCIENCE OF THE TOTAL ENVIRONMENT, 1991, 104 (03) : 191 - 209
[2] MERCURY CONTAMINATION OF THE STRIPED DOLPHIN STENELLA-COERULEOALBA MEYEN FROM THE FRENCH MEDITERRANEAN COASTS
AUGIER, H
PARK, WK
RONNEAU, C
[J]. MARINE POLLUTION BULLETIN, 1993, 26 (06) : 306 - 310
[3] COMPARATIVE-STUDIES ON CYTOTOXIC AND GENOTOXIC EFFECTS OF 2 ORGANIC MERCURY-COMPOUNDS IN LYMPHOCYTES AND GASTRIC-MUCOSA CELLS OF SPRAGUE-DAWLEY RATS
BETTI, C
BARALE, R
POOLZOBEL, BL
[J]. ENVIRONMENTAL AND MOLECULAR MUTAGENESIS, 1993, 22 (03) : 172 - 180
[4] POLYCHLORINATED BIPHENYL (PCB)-CONTAMINATED FISH INDUCES VITAMIN-A AND THYROID-HORMONE DEFICIENCY IN THE COMMON SEAL (PHOCA-VITULINA)
BROUWER, A
REIJNDERS, PJH
KOEMAN, JH
[J]. AQUATIC TOXICOLOGY, 1989, 15 (01) : 99 - 105
[5] MERCURY AND SELENIUM CONTENT AND CHEMICAL FORM IN FISH MUSCLE
CAPPON, CJ
SMITH, JC
[J]. ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY, 1981, 10 (03) : 305 - 319
[6] Cummins J.E., 1988, Ecologist, V18, P193
[7] GENOTOXICITY OF MERCURY-COMPOUNDS - A REVIEW
DEFLORA, S
BENNICELLI, C
BAGNASCO, M
[J]. MUTATION RESEARCH, 1994, 317 (01): : 57 - 79
[8] FOSSI C, 1992, MAR POLLUT B, V24, P453
[9] FOSSI C, 1994, NONDESTRUCTIVE BIOMA, P345
[10] CYTOGENETIC MONITORING OF FISHERMEN WITH ENVIRONMENTAL MERCURY EXPOSURE
FRANCHI, E
LOPRIENO, G
BALLARDIN, M
PETROZZI, L
MIGLIORE, L
[J]. MUTATION RESEARCH, 1994, 320 (1-2): : 23 - 29

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