Identification of botanicals and potential contaminants through RFLP and sequencing

被引：15

作者：

Lum, MR

Potter, E

Dang, T

Heber, D

Hardy, M

Hirsch, AM ^{[1
]}

机构：

[1] Univ Calif Los Angeles, Dept Mol Cell & Dev Biol, Los Angeles, CA 90095 USA

[2] Univ Calif Los Angeles, Sch Med, Ctr Human Nutr, Los Angeles, CA 90095 USA

[3] Univ Calif Los Angeles, Inst Mol Biol, Los Angeles, CA 90095 USA

来源：

PLANTA MEDICA | 2005年 / 71卷 / 09期

关键词：

PCR amplification; alfalfa; red clover; repair reaction; RFLP; dietary supplements;

D O I：

10.1055/s-2005-871230

中图分类号：

Q94 [植物学];

学科分类号：

071001 [植物学];

摘要：

Botanical supplements for health enhancement are being increasingly used in the United States, but no safeguards are formally in place to ensure that they are not contaminated with non-efficacious or potentially harmful plant material. A molecular approach, which allows the authentication of botanical ingredients and detection of contaminating plant material by analyzing the ITS-1 region by PCR-RFLP and subsequent sequencing, is described. When using starting material from which DNA can be obtained, this method has the potential for identifying both primary and contaminating plant material in botanical dietary supplements.

引用

页码：841 / 846

页数：6

共 19 条

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