Transforming activity of the lymphotoxin-β receptor revealed by expression screening

被引：8

作者：

Fujiwara, S

Yamashita, Y

Choi, YM

Wada, T

Kaneda, R

Takada, S

Maruyama, Y

Ozawa, K

Mano, H ^{[1
]}

机构：

[1] Jichi Med Sch, Div Funct Genom, Minami Kawachi, Tochigi 3290498, Japan

[2] Jichi Med Sch, Div Haematol, Minami Kawachi, Tochigi 3290498, Japan

[3] Fukushima Med Univ, Dept Internal Med 1, Fukushima 9601295, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2005年 / 338卷 / 02期

关键词：

lymphotoxin-beta receptor; pancreatic ductal carcinoma; retrovirus; cDNA expression library; oncogene;

D O I：

10.1016/j.bbrc.2005.10.080

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Pancreatic ductal carcinoma (PDC) remains one of the most intractable human malignancies. To obtain insight into the molecular pathogenesis of PDC we constructed a retroviral cDNA expression library with total RNA isolated from the PDC cell line MiaPaCa-2. Screening of this library with the use of a focus formation assay with NIH 3T3 mouse fibroblasts resulted in the identification of 13 independent genes with transforming activity. One of the cDNAs thus identified encodes an NH2-terminally truncated form of the lymphotoxin-beta receptor (LTBR). The transforming activity of this short-type LTBR in 3T3 cells was confirmed by both an in vitro assay of cell growth in soft agar and an in vivo assay of turnorigenicity in nude mice. The full-length (wild-type) LTBR protein was also found to manifest similar transforming activity. These observations suggest that LTBR, which belongs to the tumor necrosis factor receptor superfamily of proteins, may contribute to human carcinogenesis. (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：1256 / 1262

页数：7

共 17 条

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