Combined "dual" absorption and fluorescence smartphone spectrometers

被引:87
作者
Hossain, Md. Arafat [1 ,3 ]
Canning, John [1 ,2 ]
Ast, Sandra [2 ]
Cook, Kevin [1 ]
Rutledge, Peter J. [2 ]
Jamalipour, Abbas [3 ]
机构
[1] Univ Sydney, Sch Chem, Iinterdisciplinary Photon Labs iPL, Sydney, NSW 2006, Australia
[2] Univ Sydney, Sch Chem, Sydney, NSW 2006, Australia
[3] Univ Sydney, Sch Elect & Informat Engn, Sydney, NSW 2006, Australia
基金
澳大利亚研究理事会;
关键词
Spectrometers - Light emitting diodes - Fluorophores;
D O I
10.1364/OL.40.001737
中图分类号
O43 [光学];
学科分类号
070207 [光学];
摘要
A combined "dual" absorption and fluorescence smartphone spectrometer is demonstrated. The optical sources used in the system are the white flash LED of the smartphone and an orthogonally positioned and interchangeable UV (lambda(ex) = 370 nm) and blue (lambda(ex) = 450 nm) LED. The dispersive element is a low-cost, nano-imprinted diffraction grating coated with Au. Detection over a 300 nm span with 0.42 nm/pixel resolution was carried out with the camera CMOS chip. By integrating the blue and UV excitation sources into the white LED circuitry, the entire system is self-contained within a 3D printed case and powered from the smartphone battery; the design can be scaled to add further excitation sources. Using a customized app, acquisition of absorption and fluorescence spectra are demonstrated using a blue-absorbing and green-emitting pH-sensitive amino-naphthalimide-based fluorescent probe and a UV-absorbing and blue-emitting Zn2+-sensitive fluoro-ionophore. (C) 2015 Optical Society of America
引用
收藏
页码:1737 / 1740
页数:4
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