Isolation, mapping, and regulated expression of the gene encoding mouse C-type natriuretic peptide

被引:33
作者
Huang, HM
Acuff, CG
Steinhelper, ME
机构
[1] UNIV ALABAMA, DEPT MED, DIV CARDIOVASC DIS, VASC BIOL & HYPERTENS PROGRAM, BIRMINGHAM, AL 35294 USA
[2] UNIV ALABAMA, DEPT CELL BIOL, BIRMINGHAM, AL 35294 USA
来源
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY | 1996年 / 271卷 / 04期
关键词
fluid balance; ovary; uterus; estrous cycle; chromosome; 1;
D O I
10.1152/ajpheart.1996.271.4.H1565
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Genomic sequences encoding mouse C-type natriuretic peptide (CNP) were isolated from bacteriophage libraries and characterized by restriction enzyme and sequence analysis. The mouse CNP gene (Nppc) comprised at least two exons and one intron and included several cis-regulatory elements in the 5'-flanking sequence. The deduced amino acid sequence of mouse CNP-22 was identical to other mammalian CNPs. Analysis of allele distributions in interspecific back-cross and recombinant inbred strains assigned Nppc to chromosome 1. CNP transcripts were detected by ribonuclease protection analysis in brain, ovary, and uterus, with lower levels in testes and epididymus. Uterine CNP transcripts and protein were low in sexually immature mice and adults at estrus and increased at proestrus, but similar variations in ovarian CNP expression were not statistically significant. Atrial natriuretic peptide and B-type natriuretic peptide transcripts were not detected in mouse ovary or uterus. Thus CNP gene expression is regulated by tissue-specific and inducible mechanisms in female reproductive organs. Correlations between CNP expression and uterine fluid content suggest that CNP may regulate uterine fluid balance in mice and other mammals.
引用
收藏
页码:H1565 / H1575
页数:11
相关论文
共 36 条
[1]   A MEMBRANE FORM OF GUANYLATE-CYCLASE IS AN ATRIAL NATRIURETIC PEPTIDE RECEPTOR [J].
CHINKERS, M ;
GARBERS, DL ;
CHANG, MS ;
LOWE, DG ;
CHIN, HM ;
GOEDDEL, DV ;
SCHULZ, S .
NATURE, 1989, 338 (6210) :78-83
[2]  
CHRISMAN TD, 1993, J BIOL CHEM, V268, P3698
[3]  
Clark J.H., 1988, P675
[4]   VASCULAR ENDOTHELIAL GROWTH-FACTOR VASCULAR-PERMEABILITY FACTOR EXPRESSION IN THE RAT UTERUS - RAPID STIMULATION BY ESTROGEN CORRELATES WITH ESTROGEN-INDUCED INCREASES IN UTERINE CAPILLARY-PERMEABILITY AND GROWTH [J].
CULLINANBOVE, K ;
KOOS, RD .
ENDOCRINOLOGY, 1993, 133 (02) :829-837
[5]   CHARACTERIZATION AND DISTRIBUTION OF NATRIURETIC PEPTIDE RECEPTORS IN THE RAT UTERUS [J].
DOSREIS, AM ;
FUJIO, N ;
DAM, TV ;
MUKADDAMDAHER, S ;
JANKOWSKI, M ;
TREMBLAY, J ;
GUTKOWSKA, J .
ENDOCRINOLOGY, 1995, 136 (10) :4247-4253
[6]   C-TYPE NATRIURETIC PEPTIDE IS A GROWTH INHIBITOR OF RAT VASCULAR SMOOTH-MUSCLE CELLS [J].
FURUYA, M ;
YOSHIDA, M ;
HAYASHI, Y ;
OHNUMA, N ;
MINAMINO, N ;
KANGAWA, K ;
MATSUO, H .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1991, 177 (03) :927-931
[7]   THE ATRIAL-NATRIURETIC-PEPTIDE SYSTEM IN RAT OVARIES [J].
GUTKOWSKA, J ;
TREMBLAY, J ;
ANTAKLY, T ;
MEYER, R ;
MUKADDAMDAHER, S ;
NEMER, M .
ENDOCRINOLOGY, 1993, 132 (02) :693-700
[8]   The effect of estrogen on the permeability of the uterine capillaries [J].
Hechter, O ;
Krohn, L ;
Harris, J .
ENDOCRINOLOGY, 1941, 29 (03) :386-392
[9]   C-TYPE NATRIURETIC PEPTIDE IMMUNOREACTIVITY IN HUMAN BREAST VASCULAR ENDOTHELIAL-CELLS [J].
HEUBLEIN, DM ;
CLAVELL, AL ;
STINGO, AJ ;
LERMAN, A ;
WOLD, L ;
BURNETT, JC .
PEPTIDES, 1992, 13 (05) :1017-1019
[10]   GENETIC DECREASES IN ATRIAL-NATRIURETIC-PEPTIDE AND SALT-SENSITIVE HYPERTENSION [J].
JOHN, SWM ;
KREGE, JH ;
OLIVER, PM ;
HAGAMAN, JR ;
HODGIN, JB ;
PANG, SC ;
FLYNN, TG ;
SMITHIES, O .
SCIENCE, 1995, 267 (5198) :679-681