Macromolecular properties of cepacian in water and in dimethylsulfoxide

被引：17

作者：

Herasimenka, Yury ^{[1
]}

Cescuttia, Paola ^{[1
]}

Noguera, Carlos E. Sampaio ^{[2
]}

Ruggiero, Jose R. ^{[2
]}

Urbani, Ranieri ^{[1
]}

Impallomeni, Giuseppe ^{[3
]}

Zanetti, Flavio ^{[4
]}

Campidelli, Stphane ^{[5
]}

Prato, Maurizio ^{[5
]}

Rizzo, Roberto ^{[1
]}

机构：

[1] Univ Trieste, Dept Biochem Biophys & Macromol Chem, I-34127 Trieste, Italy

[2] Univ Sao Paulo, Dept Phys, IBILCE, Sao Jose do Rio Preto, Brazil

[3] CNR, Inst Chem & Technol Polymers, I-95125 Catania, Italy

[4] Eurand SpA, I-34012 Trieste, Italy

[5] Univ Trieste, Dept Pharmaceut Sci, I-34127 Trieste, Italy

来源：

CARBOHYDRATE RESEARCH | 2008年 / 343卷 / 01期

关键词：

exopolysaccharide; Burkholderia cepacia complex; biofilm; conformational properties; dimethylsulfoxide solutions;

D O I：

10.1016/j.carres.2007.10.003

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Cepacian is the exopolysaccharide produced by the majority of the so far investigated clinical strains of the Burkholderia cepacia complex. This is a group of nine closely related bacterial species that might cause serious lung infections in cystic fibrosis patients, in some cases leading to death. In this paper the aggregation ability and the conformational properties of cepacian chain were investigated to understand its role in biofilm formation. Viscosity and atomic force microscopy studies in water and in mixed (dimethylsulfoxide/water) solvent indicated the formation of double stranded molecular structures in aqueous solutions. Inter-residue short distances along cepacian chain were investigated by NOE NMR, which showed that two side chains of cepacian were not conformation ally free due to strong interactions with the polymer backbone. These interactions were attributed to hydrogen bonding and contributed to structure rigidity. (c) 2007 Elsevier Ltd. All rights reserved.

引用

页码：81 / 89

页数：9

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