PNA-related oligonucleotide mimics and their evaluation for nucleic acid hybridization studies and analysis

被引：21

作者：

Efimov, V ^{[1
]}

Choob, M

Buryakova, A

Phelan, D

Chakhmakhcheva, O

机构：

[1] Shemyakin & Ovchinnikov Inst Bioorgan Chem, Moscow 117871, Russia

[2] Act Motif, Carlsbad, CA 92008 USA

来源：

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS | 2001年 / 20卷 / 4-7期

关键词：

D O I：

10.1081/NCN-100002316

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

DNA mimics containing phosphonate analogues of PNAs (pPNAs), particularly PNA-pPNA hybrids as well as hetero-oligomers consisted of pPNA units and PNA-like molecules on the base of trans-4-hydroxy-L-proline (HypNA) have been synthesized. The evaluation of their effectiveness, in assays based on the hybridization technique in the comparison with natural oligonucleotides and classical PNAs has shown a high potential of these mimics as sensor molecules for nucleic acid based diagnostics and as molecular probes for mRNA isolation.

引用

页码：419 / 428

页数：10

共 17 条

[1] SOLID-PHASE SYNTHESIS OF DIRECTLY LINKED PNA-DNA-HYBRIDS [J].

BERGMANN, F ;

BANNWARTH, W ;

TAM, S .

TETRAHEDRON LETTERS, 1995, 36 (38) :6823-6826

[2]

EFIMOV V, 2000, IN PRESS BIOORGAN KH, V26

[3]

EFIMOV V, 2000, IN PRESS SOLID PHASE

[4] Synthesis of polyacrylamides N-substituted with PNA-like oligonucleotide mimics for molecular diagnostic applications [J].

Efimov, VA ;

Buryakova, AA ;

Chakhmakhcheva, OG .

NUCLEIC ACIDS RESEARCH, 1999, 27 (22) :4416-4426

[5]

Efimov VA, 1999, BIOORG KHIM+, V25, P611

[6]

Efimov VA, 1999, BIOORG KHIM+, V25, P848

[7] Synthesis and evaluation of some properties of chimeric oligomers containing PNA and phosphono-PNA residues [J].

Efimov, VA ;

Choob, MV ;

Buryakova, AA ;

Kalinkina, AL ;

Chakhmakhcheva, OG .

NUCLEIC ACIDS RESEARCH, 1998, 26 (02) :566-575

[8]

Efimov VA, 1998, BIOORG KHIM+, V24, P696

[9] PNA HYBRIDIZES TO COMPLEMENTARY OLIGONUCLEOTIDES OBEYING THE WATSON-CRICK HYDROGEN-BONDING RULES [J].

EGHOLM, M ;

BUCHARDT, O ;

CHRISTENSEN, L ;

BEHRENS, C ;

FREIER, SM ;

DRIVER, DA ;

BERG, RH ;

KIM, SK ;

NORDEN, B ;

NIELSEN, PE .

NATURE, 1993, 365 (6446) :566-568

[10] PNA array technology in molecular diagnostics [J].

Geiger, A ;

Lester, A ;

Kleiber, J ;

Orum, H .

NUCLEOSIDES & NUCLEOTIDES, 1998, 17 (9-11) :1717-1724

← 1 2 →