Metabolic footprint of Lactobacillus acidophilus NCFM at different pH

被引：11

作者：

Sulek, Karolina ^{[1
]}

Frandsen, Henrik Lauritz ^{[2
]}

Smedsgaard, Jorn ^{[2
]}

Skov, Thomas Hjort ^{[3
]}

Wilcks, Andrea ^{[1
]}

Licht, Tine Rask ^{[1
]}

机构：

[1] Tech Univ Denmark, Div Microbiol & Risk Assessment, DTU Food, DK-2860 Soborg, Denmark

[2] Tech Univ Denmark, Div Food Chem, DTU Food, DK-2860 Soborg, Denmark

[3] Univ Copenhagen, Div Qual & Technol, Dept Food Sci, Fac Life Sci, DK-1958 Frederiksberg C, Denmark

来源：

METABOLOMICS | 2012年 / 8卷 / 02期

关键词：

Lactobacillus acidophilus NCFM; Metabolomics; Metabolite footprint; GIT; Probiotics; LCMS; LACTIC-ACID BACTERIA; GENES; MICROBIOME; MICROFLORA; PLANTARUM; GENOMICS;

D O I：

10.1007/s11306-011-0305-4

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Lactobacillus acidophilus NCFM is a well known microorganism from the genomic and probiotic point of view. In order to analyze the potential interactions of NCFM with the surrounding environment, in vitro tests with the metabolic footprinting approach were performed. It was found that NCFM increased the concentration of lactic acid, succinic acid, adenine and arginine in the medium. The metabolism of NCFM did not change significantly between pH 5 and 7, suggesting that other environmental factors than pH might have bigger impact on its colonization throughout the gastrointestinal tract.

引用

页码：244 / 252

页数：9

共 33 条

[1] Complete genome sequence of the probiotic lactic acid bacterium Lactobacillus acidophilus NCFM [J].

Altermann, E ;

Russell, WM ;

Azcarate-Peril, MA ;

Barrangou, R ;

Buck, BL ;

McAuliffe, O ;

Souther, N ;

Dobson, A ;

Duong, T ;

Callanan, M ;

Lick, S ;

Hamrick, A ;

Cano, R ;

Klaenhammer, TR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (11) :3906-3912

[2] Functional and comparative genomic analyses of an operon involved in fructooligosaccharide utilization by Lactobacillus acidophilus [J].

Barrangou, R ;

Altermann, E ;

Hutkins, R ;

Cano, R ;

Klaenhammer, TR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (15) :8957-8962

[3] Bacterial linguistic communication and social intelligence [J].

Ben Jacob, E ;

Becker, I ;

Shapira, Y ;

Levine, H .

TRENDS IN MICROBIOLOGY, 2004, 12 (08) :366-372

[4]

Berg J., 2001, Biochemistry, V5th

[5] Identification of Lactobacillus plantarum genes that are induced in the gastrointestinal tract of mice [J].

Bron, PA ;

Grangette, C ;

Mercenier, A ;

de Vos, WM ;

Kleerebezem, M .

JOURNAL OF BACTERIOLOGY, 2004, 186 (17) :5721-5729

[6] Selection and characterization of conditionally active promoters in Lactobacillus plantarum, using alanine racemase as a promoter probe [J].

Bron, PA ;

Hoffer, SM ;

Van Swam, II ;

De Vos, WM ;

Kleerebezem, M .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2004, 70 (01) :310-317

[7] Arginine catabolism by sourdough lactic acid bacteria:: Purification and characterization of the arginine deiminase pathway enzymes from Lactobacillus sanfranciscensis CB1 [J].

De Angelis, M ;

Mariotti, L ;

Rossi, J ;

Servili, M ;

Fox, PF ;

Rollán, G ;

Gobbetti, M .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2002, 68 (12) :6193-6201

[8] Post-genomics of lactic acid bacteria and other food-grade bacteria to discover gut functionaility [J].

de Vos, WM ;

Bron, PA ;

Kleerebezem, M .

CURRENT OPINION IN BIOTECHNOLOGY, 2004, 15 (02) :86-93

[9] Growth requirements of Lactobacillus johnsonii in skim and UHT milk [J].

Elli, M ;

Zink, R ;

Reniero, R ;

Morelli, L .

INTERNATIONAL DAIRY JOURNAL, 1999, 9 (08) :507-513

[10] FACTORS INVOLVED IN ADHERENCE OF LACTOBACILLI TO HUMAN CACO-2 CELLS [J].

GREENE, JD ;

KLAENHAMMER, TR .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1994, 60 (12) :4487-4494

← 1 2 3 4 →