Characterisation of VanA and VanB elements from glycopeptide-resistant Enterococcus faecium from Greece

被引:12
作者
Demertzi, E
Palepou, MFI
Kaufmann, ME
Avlamis, A
Woodford, N
机构
[1] Cent Publ Hlth Lab, Antibiot Resistance Monitoring & Reference Lab, London NW9 5HT, England
[2] Cent Publ Hlth Lab, Lab Hosp Infect, London NW9 5HT, England
[3] Laikon Gen Hosp, Dept Microbiol, Athens, Greece
关键词
D O I
10.1099/0022-1317-50-8-682
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Ten glycopeptide-resistant Enterococcus faecium isolates from separate patients in Laikon General Hospital, Athens were studied. Eight isolates had the VanA phenotype and represented variants of three strains based on SmaI macrorestriction banding patterns. Their VanA elements were compared with the prototype element, Tn1546, by an overlapping PCR method. Three related isolates contained resistance elements indistinguishable from Tn1546 (designated Greek type I). The other five isolates all contained identical elements that differed from Tn1546 by the presence of IS1251 between vanS and vanH, by a point mutation (G -->T) at nucleotide position 8234 within vanX and by a partial loss of transposition gene orf1 (designated Greek type II). Two distinct strains of E. faecium with the VanB phenotype were obtained. HhaI digestion of an amplified fragment of the vanB gene indicated that both strains contained the vanB2 allele, and further PCR assays confirmed that the vanB2 gene cluster was located within a Tn5382-like element.
引用
收藏
页码:682 / 687
页数:6
相关论文
共 27 条
[1]   CHARACTERIZATION OF TN1546, A TN3-RELATED TRANSPOSON CONFERRING GLYCOPEPTIDE RESISTANCE BY SYNTHESIS OF DEPSIPEPTIDE PEPTIDOGLYCAN PRECURSORS IN ENTEROCOCCUS-FAECIUM BM4147 [J].
ARTHUR, M ;
MOLINAS, C ;
DEPARDIEU, F ;
COURVALIN, P .
JOURNAL OF BACTERIOLOGY, 1993, 175 (01) :117-127
[2]   Genetic linkage and cotransfer of a novel, vanB-containing transposon (Tn5382) and a low-affinity penicillin-binding protein 5 gene in a clinical vancomycin-resistant Enterococcus faecium isolate [J].
Carias, LL ;
Rudin, SD ;
Donskey, CJ ;
Rice, LB .
JOURNAL OF BACTERIOLOGY, 1998, 180 (17) :4426-4434
[3]   Heterogeneity in the vanB gene cluster of genomically diverse clinical strains of vancomycin-resistant enterococci [J].
Dahl, KH ;
Simonsen, GS ;
Olsvik, O ;
Sundsfjord, A .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1999, 43 (05) :1105-1110
[4]   Genetic linkage of the vanB2 gene cluster to Tn5382 in vancomycin-resistant enterococci and characterization of two novel insertion sequences [J].
Dahl, KH ;
Lundblad, EW ;
Rokenes, TP ;
Olsvik, O ;
Sundsfjord, A .
MICROBIOLOGY-SGM, 2000, 146 :1469-1479
[5]  
Darini ALD, 1999, FEMS MICROBIOL LETT, V173, P341
[6]   DETECTION OF GLYCOPEPTIDE RESISTANCE GENOTYPES AND IDENTIFICATION TO THE SPECIES LEVEL OF CLINICALLY RELEVANT ENTEROCOCCI BY PCR [J].
DUTKAMALEN, S ;
EVERS, S ;
COURVALIN, P .
JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (01) :24-27
[7]  
DUTKAMALEN S, 1995, J CLIN MICROBIOL, V1434, P95
[8]   VanE, a new type of acquired glycopeptide resistance in Enterococcus faecalis BM4405 [J].
Fines, M ;
Perichon, B ;
Reynolds, P ;
Sahm, DF ;
Courvalin, P .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1999, 43 (09) :2161-2164
[9]   Characterization of transposon Tn1549, conferring VanB-type resistance in Enterococcus spp. [J].
Garnier, F ;
Taourit, S ;
Glaser, P ;
Courvalin, P ;
Galimand, M .
MICROBIOLOGY-UK, 2000, 146 :1481-1489
[10]   A GENE CONFERRING RESISTANCE TO VANCOMYCIN BUT NOT TEICOPLANIN IN ISOLATES OF ENTEROCOCCUS-FAECALIS AND ENTEROCOCCUS-FAECIUM DEMONSTRATES HOMOLOGY WITH VANB, VANA, AND VANC GENES OF ENTEROCOCCI [J].
GOLD, HS ;
UNAL, S ;
CERCENADO, E ;
THAUVINELIOPOULOS, C ;
ELIOPOULOS, GM ;
WENNERSTEN, CB ;
MOELLERING, RC .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1993, 37 (08) :1604-1609