Investigation and interpretation of band broadening in size exclusion chromatography

被引：51

作者：

Busnel, JP ^{[1
]}

Foucault, F

Denis, L

Lee, W

Chang, T

机构：

[1] Univ Maine, Fac Sci, F-72085 Le Mans 9, France

[2] POSTECH, Dept Chem, Pohang 790784, South Korea

来源：

JOURNAL OF CHROMATOGRAPHY A | 2001年 / 930卷 / 1-2期

关键词：

band broadening; temperature-gradient interaction chromatography; peak shape; exponentially modified Gaussian functions;

D O I：

10.1016/S0021-9673(01)01159-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Study of Band Broadening occurring in Size Exclusion Chromatography (SEC) is reported using very narrow PS standards obtained and characterised by Temperature Gradient Interaction Chromatography (TGIC). Chromatograms are fitted by Exponentially Modified Gaussian functions (EMG) and mapping of band broadening is obtained for different column sets. Interpretation of the skewing of the chromatograms is proposed with a new model using Brownian motion properties inside the pores. That explains why band broadening and tailing become so important near total exclusion volume. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：61 / 71

页数：11

共 18 条

[1] POLYMER REACTORS AND MOLECULAR WEIGHT DISTRIBUTION .8. A METHOD OF INTERPRETING SKEWED GPC CHROMATOGRAMS [J].