Detection of H5N1 avian influenza virus from mosquitoes collected in an infected poultry farm in Thailand

被引：29

作者：

Barbazan, Philippe ^{[1
,2
]}

Thitithanyanont, Arunee ^{[1
,3
]}

Misse, Dorothee ^{[2
,4
]}

Dubot, Audrey ^{[1
,2
]}

Bosc, Priscille ^{[5
]}

Luangsri, Natsuang ^{[1
]}

Gonzalez, Jean-Paul ^{[1
,2
]}

Kittayapong, Pattamaporn ^{[1
,6
]}

机构：

[1] Mahidol Univ Salaya, Fac Sci, Ctr Excellence Vectors & Vector Borne Dis, Nakhon Pathom 73170, Thailand

[2] Inst Rech Dev, IRD UR 178, Paris, France

[3] Mahidol Univ, Fac Sci, Dept Microbiol, Bangkok 10700, Thailand

[4] CNRS, IRD 2724 Genet & Evolut Malad Infect, GEMI UMR, Montpellier, France

[5] ApoH Technol, IRD, Montpellier, France

[6] Mahidol Univ, Fac Sci, Dept Biol, Bangkok 10700, Thailand

来源：

VECTOR-BORNE AND ZOONOTIC DISEASES | 2008年 / 8卷 / 01期

关键词：

H5N1; surveillance; detection; wild; animals; poultry; mosquito; mosquito blood meal;

D O I：

10.1089/vbz.2007.0142

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

Blood-engorged mosquitoes were collected at poultry farms during an outbreak of highly pathogenic avian influenza in Central Thailand during October 2005. These mosquitoes tested positive for H5N1 virus by reverse transcription-polymerase chain reaction (RT-PCR). Results were confirmed by limited sequencing of the H5 and N1 segments. Infection and replication of this virus in the C6/36 mosquito cell line was confirmed by quantitative real-time PCR. However, transmission by mosquitoes was not evaluated, and further research is needed. Collecting and testing mosquitoes engorged with the blood of domestic or wild animals could be a valuable tool for veterinary and public health authorities who conduct surveillance for H5N1 virus spread.

引用

页码：105 / 109

页数：5

共 18 条

[1] An overview of the epidemiology of avian influenza [J].