Sugar transport through channels reconstituted in planar lipid membranes

被引：18

作者：

Winterhalter, M ^{[1
]}

机构：

[1] Univ Basel, Bioctr, CH-4056 Basel, Switzerland

来源：

COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS | 1999年 / 149卷 / 1-3期

关键词：

lipid membranes; sugar transport; power spectrum of ion current;

D O I：

10.1016/S0927-7757(98)00290-8

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

We have reconstituted maltoporin, a maltose-specific transmembrane protein, into planar lipid membranes and recorded the power spectrum of the ion current after application of a small transmembrane voltage. The presence of maltose in the aqueous phase causes blocking of the channels in a concentration-dependent manner. The power spectra of the ion current show, in addition to the 1/f dependence, a pronounced Lorentzian with a corner frequency proportional to the maltose concentration. A series of measurements with different maltose concentrations yields the on and off rate of maltose to the channel. Under simplified conditions this allows an estimate of sugar translation. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：547 / 551

页数：5

共 11 条

[1] EVALUATION OF THE RATE CONSTANTS OF SUGAR-TRANSPORT THROUGH MALTOPORIN (LAMB) OF ESCHERICHIA-COLI FROM THE SUGAR-INDUCED CURRENT NOISE [J].