Dietary conjugated linoleic acid influences the immune response of young and old C57BL/6NCrlBR mice

被引:163
作者
Hayek, MG
Han, SN
Wu, DY
Watkins, BA
Meydani, M
Dorsey, JL
Smith, DE
Meydani, SN [1 ]
机构
[1] Tufts Univ, USDA, Jean Mayer Human Nutr Res Ctr Aging, Nutr Immunol Lab, Boston, MA 02111 USA
[2] Purdue Univ, Dept Food Sci, Lipid Chem & Metab Lab, W Lafayette, IN 47907 USA
[3] Florida State Univ, Dept Nutr Food & Movement Sci, Tallahassee, FL 32306 USA
关键词
conjugated linoleic acid; mice; immune response; age;
D O I
10.1093/jn/129.1.32
中图分类号
R15 [营养卫生、食品卫生]; TS201 [基础科学];
学科分类号
100403 ;
摘要
Aging is associated with a decline in the immune response in mammals. Conjugated linoleic acid (CLA) has been suggested to have immunoenhancing properties. We examined the influence of dietary CLA on the immune response of young and old mice. Forty young (4 mo) and 40 old (22 mo) mice consumed ad libitum diets containing 0 or 1 g CLA /100 g for 8 wk. Splenocytes from half of the mice were isolated to evaluate proliferation to concanavalin A (Con A) (0.5, 1.5, 5.0 mg/L) and phytohemagglutinin A (PHA) (5, 20, 40 mg/L) and lipopolysaccharide (LPS) (5, 15, 30 mg/L), natural killer cell (NK) activity and prostaglandin (PG)E-2 and interleukin (IL)-2 production. The remaining mice were used to evaluate in vivo delayed-type hypersensitivity (DTH) skin response. There was a significant decline due to age in response to all three mitogens tested (P < 0.05). CLA supplementation significantly increased all CLA isomers measured in hepatic neutral lipids and phospholipids (P < 0.05). Young mice fed 1% CLA had greater splenocyte proliferation in response to Con A (0.5 and 5.0 mg/L) and PHA (40 mg/L) (P < 0.05) than young mice fed control diet. Old mice fed 1 g CLA/100 g had significantly higher proliferative response to optimal concentrations of Con A (1.5 mg/L) (P < 0.001) than the mice fed the control diet. Old mice fed the control diet had significantly lower splenocyte IL-2 production than the young mice (P < 0.005). CLA-supplemented young mice had significantly higher splenocyte IL-2 production than those fed the control diet (P < 0.05). CLA had no effect on NK cell activity, PGE(2) production or DTH in young or old mice. Further studies ave needed to determine the mechanism of CLA-induced enhancement of IL-2 production and T cell proliferation.
引用
收藏
页码:32 / 38
页数:7
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