Characterization and sequence analysis of the replicator region of the novel plasmid pALC1 from Paracoccus alcaliphilus

被引:13
作者
Bartosik, D [1 ]
Witkowska, M [1 ]
Baj, J [1 ]
Wlodarczyk, M [1 ]
机构
[1] Warsaw Univ, Inst Microbiol, Dept Bacterial Genet, PL-02096 Warsaw, Poland
关键词
plasmid pALC1; Paracoccus alcaliphilus; replicator region; iterons;
D O I
10.1006/plas.2000.1505
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
The replicator region of a low-copy-number plasmid, pALC1. of Paracoccus alcaliphilus JCM 7364 was cloned in a form of the minireplicon pALC100 (3.6 bb). The host range of the minireplicon embraces several species of genus Paracoccus, as well as Agrobacterium tumefaciens leguminosarum and Rhodobacter sphaeroides (all belonging to alpha -Proteoobacteria), but not Escherichia coli. The complete nucleotide sequence of the replicator region (2276 bp) revealed the presence of one complete open reading frame coiling for the 28.4-kDa protein (RrpA) with similarity to replication proteins of plasmid pSW500 of Erwinia stewartii and pVS1 of Pseudomonas fluorescens The iteron-like region was identified upstream of the repA gene and consisted of two clusters, of repeated sequences (17 bp long) separated by a putative DnaA box. Analysis of the predicted amino acid sequence of two adjacent incomplete ORFs suggests the localization of repA between genes involved in conjugation (traG) and partitioning (parA) within the pALC1 genome. (C) 2001 Academic Press.
引用
收藏
页码:222 / 226
页数:5
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