Evaluation of a new reagent strip rapid urease test for detection of, Helicobacter pylori infection

被引:36
作者
Yousfi, MM
ElZimaity, HMT
Genta, RM
Graham, DY
机构
[1] VET AFFAIRS MED CTR, DEPT MED, HOUSTON, TX 77030 USA
[2] VET AFFAIRS MED CTR, DEPT PATHOL, HOUSTON, TX 77030 USA
[3] BAYLOR COLL MED, DIV MOL VIROL, HOUSTON, TX 77030 USA
关键词
D O I
10.1016/S0016-5107(96)70001-9
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Background: Rapid urease tests are commonly used as a convenient method to detect Helicobacter pylori infection. Our previous experiments demonstrated enhanced efficacy of agar gel rapid urease test compared with reagent strip rapid urease tests. We evaluated the efficacy of PyloriTek, a new reagent strip rapid test for detecting H. pylori infection. Methods: Gastric antral mucosal biopsy specimens were obtained for comparison between agar gel rapid urease tests and PyloriTek (200 specimens). The rapid urease test to be used first was selected randomly. H. pylori status was determined using the Genta stain. Culture was performed to confirm H. pylori status when false rapid urease tests were suspected. Results: One hundred patients were studied; 68 had H. pylori infection. There were two false-negative and one false-positive PyloriTek when scored at 1 hour, compared with only one false-positive and no false-negative tests at 2 hours. With the agar gel rapid urease tests, there were no false-positive tests and 5 false-negative tests when scored at 1 hour, 2 false-negative tests at 12 hours and 1 at 24 hours; there were no false-positive tests. At 1 hour, 3% (95% CI = 1% to 9%) of PyloriTek tests had an erroneous categorization of H. pylori status compared with 5% for the agar gel rapid urease tests (95% CI = 1.6% to 11%) (P > 0.7). Conclusion: The new reagent strip rapid urease test, PyloriTek, is rapid and comparable in accuracy to agar gel rapid urease tests for detecting H. pylori infection.
引用
收藏
页码:519 / 522
页数:4
相关论文
共 37 条
[1]   RAPID DETECTION OF GASTRIC CAMPYLOBACTER-PYLORI COLONIZATION BY A SIMPLE BIOCHEMICAL TEST [J].
ABDALLA, S ;
MARCO, F ;
PEREZ, RM ;
PIQUE, JM ;
BORDAS, JM ;
DEANTA, MTJ ;
TERES, J .
JOURNAL OF CLINICAL MICROBIOLOGY, 1989, 27 (11) :2604-2605
[2]  
ALPERT LC, 1989, EUR J GASTROEN HEPAT, V1, P17
[3]  
BARTHEL JS, 1990, REV INFECT DIS, V12, pS107
[4]  
BROWN KE, 1993, GASTROENTEROL CLIN N, V22, P105
[5]  
CHODOS JE, 1988, AM J GASTROENTEROL, V83, P1226
[6]  
CONTINIBALI S, 1990, AM J GASTROENTEROL, V85, P1573
[7]   COMPARISON OF RAPID UREASE TESTS, STAINING TECHNIQUES, AND GROWTH ON DIFFERENT SOLID MEDIA FOR DETECTION OF CAMPYLOBACTER-PYLORI [J].
COUDRON, PE ;
KIRBY, DF .
JOURNAL OF CLINICAL MICROBIOLOGY, 1989, 27 (07) :1527-1530
[8]   ACCURACY OF INVASIVE AND NONINVASIVE TESTS TO DIAGNOSE HELICOBACTER-PYLORI INFECTION [J].
CUTLER, AF ;
HAVSTAD, S ;
MA, CK ;
BLASER, MJ ;
PEREZPEREZ, GI ;
SCHUBERT, TT .
GASTROENTEROLOGY, 1995, 109 (01) :136-141
[9]   THE RELIABILITY OF UREASE TESTS, HISTOLOGY AND CULTURE IN THE DIAGNOSIS OF CAMPYLOBACTER-PYLORI INFECTION [J].
DELTENRE, M ;
GLUPCZYNSKI, Y ;
DEPREZ, C ;
NYST, JF ;
BURETTE, A ;
LABBE, M ;
JONAS, C ;
DEKOSTER, E .
SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY, 1989, 24 :19-24
[10]  
ELZIMAITY HMT, 1995, AM J GASTROENTEROL, V90, P1962