Regulation of stem cell maintenance and transit amplifying cell proliferation by TGF-β signaling in Drosophila spermatogenesis

被引:175
作者
Shivdasani, AA [1 ]
Ingham, PW [1 ]
机构
[1] Univ Sheffield, Dept Biomed Sci, Ctr Dev Genet, MRC,Intercellular Signalling Grp, Sheffield S10 2TN, S Yorkshire, England
基金
英国惠康基金; 英国医学研究理事会;
关键词
D O I
10.1016/j.cub.2003.10.063
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The continuous and steady supply of transient cell types such as skin, blood and gut depends crucially on the controlled proliferation of stem cells and their transit amplifying progeny. Although it is thought that signaling to and from support cells might play a key role in these processes, few signals that might mediate this interaction have been identified. During spermatogenesis in Drosophila, the asymmetric division of each germ line stem cell results in its self-renewal and the production of a committed progenitor that undergoes four mitotic divisions before differentiating while remaining in intimate contact with somatic support cells [1]. Previous data have suggested that TGF-beta signaling pathway components punt and schnurri are required in the somatic support cells to restrict germ cell proliferation [2]. Here, by contrast, we show that the maintenance and proliferation of germ line stem cells and their progeny depends upon their ability to transduce the activity of a somatically expressed TGF-beta ligand, the BMP5/8 ortholog Glass Bottom Boat. We further demonstrate that TGF-beta signaling represses the expression of the Barn protein, which is both necessary and sufficient for germ cell differentiation, thereby maintaining germ line stem cells and spermatogonia in their proliferative state.
引用
收藏
页码:2065 / 2072
页数:8
相关论文
共 32 条
  • [1] THE DROSOPHILA SCHNURRI GENE ACTS IN THE DPP/TGF-BETA SIGNALING PATHWAY AND ENCODES A TRANSCRIPTION FACTOR HOMOLOGOUS TO THE HUMAN MBP FAMILY
    ARORA, K
    DAI, H
    KAZUKO, SG
    JAMAL, J
    OCONNOR, MB
    LETSOU, A
    WARRIOR, R
    [J]. CELL, 1995, 81 (05) : 781 - 790
  • [2] THE SCREW GENE ENCODES A UBIQUITOUSLY EXPRESSED MEMBER OF THE TGF-BETA FAMILY REQUIRED FOR SPECIFICATION OF DORSAL CELL FATES IN THE DROSOPHILA EMBRYO
    ARORA, K
    LEVINE, MS
    OCONNOR, MB
    [J]. GENES & DEVELOPMENT, 1994, 8 (21) : 2588 - 2601
  • [3] BRAND AH, 1993, DEVELOPMENT, V118, P401
  • [4] CHARACTERIZATION AND RELATIONSHIP OF DPP RECEPTORS ENCODED BY THE SAXOPHONE AND THICK VEINS GENES IN DROSOPHILA
    BRUMMEL, TJ
    TWOMBLY, V
    MARQUES, G
    WRANA, JL
    NEWFELD, SJ
    ATTISANO, L
    MASSAGUE, J
    OCONNOR, MB
    GELBART, WM
    [J]. CELL, 1994, 78 (02) : 251 - 261
  • [5] Transducing the Dpp morphogen gradient in the wing of Drosophila:: Regulation of Dpp targets by brinker
    Campbell, G
    Tomlinson, A
    [J]. CELL, 1999, 96 (04) : 553 - 562
  • [6] Fuller Margaret T., 1993, P71
  • [7] Gonczy P, 1997, DEVELOPMENT, V124, P4361
  • [8] Haerry TE, 1998, DEVELOPMENT, V125, P3977
  • [9] A PROTEIN-COMPONENT OF DROSOPHILA POLAR GRANULES IS ENCODED BY VASA AND HAS EXTENSIVE SEQUENCE SIMILARITY TO ATP-DEPENDENT HELICASES
    HAY, B
    JAN, LY
    JAN, YN
    [J]. CELL, 1988, 55 (04) : 577 - 587
  • [10] Transforming growth factor-beta-related genes in Drosophila and vertebrate development
    Hoffmann, F. Michael
    [J]. CURRENT OPINION IN CELL BIOLOGY, 1991, 3 (06) : 947 - 952