Production of lipase by immobilized cells of Aspergillus niger

被引：96

作者：

Ellaiah, P ^{[1
]}

Prabhakar, T ^{[1
]}

Ramakrishna, B ^{[1
]}

Taleb, AT ^{[1
]}

Adinarayana, K ^{[1
]}

机构：

[1] Andhra Univ, Pharmaceut Biotechnol Div, Dept Pharmaceut Sci, Visakhapatnam 530003, Andhra Pradesh, India

来源：

PROCESS BIOCHEMISTRY | 2004年 / 39卷 / 05期

关键词：

lipase; production; Aspergillus niger; immobilization; alginate; kappa-carrageenan; polyacrylamide;

D O I：

10.1016/S0032-9592(01)00340-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Different materials viz. alginate, K-carrageenan and polyacrylamide gel were examined for immobilization of whole cells of Aspergillus niger (ANT 90). The enzyme activity of whole cells immobilised in K-carrageenan and polyacrylamide gel was low in comparison to these immobilized with alginate. Different concentrations of alginate were tried to study their effect on lipase production. Maximum production was observed with 3% alginate and beads with 60 min curing time (4230 U/l). Olive oil was shown to increase lipase production in both free and immobilized cells. (C) 2004 Published by Elsevier Ltd.

引用

页码：525 / 528

页数：4

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