To knockout in 129 or in C57BL/6: that is the question

被引：94

作者：

Seong, E

Saunders, TL

Stewart, CL

Burmeister, M ^{[1
]}

机构：

[1] Univ Michigan, Mental Hlth Res Inst, Ann Arbor, MI 48109 USA

[2] Univ Michigan, Neurosci Program, Ann Arbor, MI 48109 USA

[3] Univ Michigan, Dept Internal Med, Ann Arbor, MI 48109 USA

[4] NCI, Canc & Dev Biol Lab, Frederick, MD 21701 USA

[5] Univ Michigan, Dept Psychiat, Ann Arbor, MI 48109 USA

[6] Univ Michigan, Dept Human Genet, Ann Arbor, MI 48109 USA

来源：

TRENDS IN GENETICS | 2004年 / 20卷 / 02期

关键词：

D O I：

10.1016/j.tig.2003.12.006

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Traditionally, knockout experiments are performed in ES cells derived from the 129 mouse strain, followed by backcrossing with the more robust C57BL/6 strain. C57BL/6-derived ES cells have only occasionally been used in this process. We compared C57BL/6- with 129-derived ES cells directly and reviewed the literature. We found that, although some steps are less efficient, the advantages of C57BL/6 mice more than compensate for these drawbacks.

引用

页码：59 / 62

页数：4

共 18 条

[1] Establishment and chimera analysis of 129/SvEv- and C57BL/6-derived mouse embryonic stem cell lines [J].