A small-scale calorimetric reactor system combined with several chemical sensors for the investigation of microbial growth processes

被引：8

作者：

Huettl, R. ^{[1
]}

Harmel, J. ^{[1
]}

Lissner, A. ^{[1
]}

Wolf, G. ^{[1
]}

Klare, P. ^{[2
]}

Vonau, W. ^{[3
]}

Berthold, F. ^{[3
]}

Herrmann, S. ^{[3
]}

机构：

[1] Tech Univ Bergakad Freiberg, Inst Phys Chem, D-09596 Freiberg, Germany

[2] IMM Ingenieurburo, Mittweida, Germany

[3] Kurt Schwabe Inst Mess & Sensortech eV Meinsberg, Ziegra Knobelsdorf, Germany

来源：

ENGINEERING IN LIFE SCIENCES | 2008年 / 8卷 / 01期

关键词：

biocalorimetry; calorimetric reactor system; electrochemical sensors; fermentation;

D O I：

10.1002/elsc.200720230

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A miniaturized reactor system based on a small-scale calorimeter (25 mL vessel) equipped with different chemical sensors is presented in this contribution. Apart from the sensor for the detection of the thermal power, the system was equipped with two chemical sensors. In comparison to established calorimeters, this newly designed combination as a whole significantly extends the range of application. The miniaturized chemical sensors can be chosen from a pool of potentiometric and amperometric ones, measuring pH value, redox potential, nitrate and glucose concentrations, as well as dissolved carbon dioxide and oxygen content. The miniaturized sensors were developed by the Kurt-Schwabe-Institute (Meinsberg) and the electrical circuit of the system was designed by IMM (Mittweida). The reactor was tested for batch cultivations using Paracoccus pantotrophus DSM 65 as a model culture.

引用

页码：56 / 61

页数：6

共 13 条

[1]

HELD K, 2006, Patent No. 20060607

[2]

HELD K, 2006, Patent No. 1020060131177

[3] Application of bench-scale biocalorimetry to photoautotrophic cultures [J].

Janssen, M ;

Patiño, R ;

von Stockar, U .

THERMOCHIMICA ACTA, 2005, 435 (01) :18-27

[4] Redefining Paracoccus denitrificans and Paracoccus pantotrophus and the case for a reassessment of the strains held by international culture collections [J].

Kelly, Donovan P. ;

Euzeby, Jean P. ;

Goodhew, Celia F. ;

Wood, Ann P. .

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2006, 56 :2495-2500

[5] Real time insights into bioprocesses using calorimetry: State of the art and potential [J].

Maskow, T. ;

Harms, H. .

ENGINEERING IN LIFE SCIENCES, 2006, 6 (03) :266-277

[6] Chip calorimetry for the monitoring of whole cell biotransformation [J].

Maskow, T ;

Lerchner, J ;

Peitzsch, M ;

Harms, H ;

Wolf, G .

JOURNAL OF BIOTECHNOLOGY, 2006, 122 (04) :431-442

[7] Control of continuous polyhydroxybutyrate synthesis using calorimetry and flow cytometry [J].

Maskow, T ;

Müller, S ;

Lösche, A ;

Harms, H ;

Kemp, R .

BIOTECHNOLOGY AND BIOENGINEERING, 2006, 93 (03) :541-552

[8] Development of biocalorimetry as a technique for process monitoring and control in technical scale fermentations [J].

Türker, M .

THERMOCHIMICA ACTA, 2004, 419 (1-2) :73-81

[9] Measurement of metabolic heat in a production-scale bioreactor by continuous and dynamic calorimetry [J].

Türker, M .

CHEMICAL ENGINEERING COMMUNICATIONS, 2003, 190 (5-8) :573-598

[10] Performance of calorimetric methods for the investigation of microbial systems in combination with additional sensors [J].

Ullrich, F ;

Winkelmann, M ;

Hüttl, R ;

Wolf, G .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2005, 383 (05) :747-751

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