Sequence-specific label-free DNA sensors based on silicon nanowires

被引：623

作者：

Li, Z ^{[1
]}

Chen, Y ^{[1
]}

Li, X ^{[1
]}

Kamins, TI ^{[1
]}

Nauka, K ^{[1
]}

Williams, RS ^{[1
]}

机构：

[1] Hewlett Packard Labs, Palo Alto, CA 94304 USA

来源：

NANO LETTERS | 2004年 / 4卷 / 02期

关键词：

D O I：

10.1021/nl034958e

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Highly sensitive and sequence-specific DNA sensors were fabricated based on silicon nanowires (SiNWs) with single stranded (ss) probe DNA molecules covalently immobilized on the nanowire surfaces, Label-free complementary (target) ss-DNA in sample solutions were recognized when the target DNA was hybridized with the probe DNA attached on the SiNW surfaces, producing a change of the conductance of the SiNWs. For a 12-mer oligonucletide probe, 25 pM of target DNA in solution was detected easily (signal/noise ratio > 6), whereas 12-mers with one base mismatch did not produce a signal above the background noise.

引用

页码：245 / 247

页数：3

共 15 条

[1] Versatile derivatisation of solid support media for covalent bonding on DNA-microchips [J].