Sequence-based typing of adeB as a potential tool to identify intraspecific groups among clinical strains of multidrug-resistant Acinetobacter baumannii

被引：23

作者：

Huys, G

Cnockaert, M

Nemec, A

Swings, J

机构：

[1] Univ Ghent, Microbiol Lab, B-9000 Ghent, Belgium

[2] Univ Ghent, BCCM, LMG, Bacteria Collect, B-9000 Ghent, Belgium

[3] Charles Univ Prague, Fac Med 3, Dept Med Microbiol, Prague, Czech Republic

[4] Charles Univ Prague, Fac Med 3, Natl Publ Hlth Inst, Prague, Czech Republic

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2005年 / 43卷 / 10期

关键词：

D O I：

10.1128/JCM.43.10.5327-5331.2005

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Sequence analysis of an 850-bp fragment internal to the aspecific drug efflux gene adeB revealed I I sequence types (STs) among a collection of 50 multidrug-resistant Acinetohacter baumannii (MDRAB) strains, including members of pan-European clones I, II, and III. The delineation of STs conformed with the intraspecific grouping of these strains previously determined by different DNA fingerprinting methods. Larger strain collections need to be screened to further explore the potential of sequence-based adeB typing as a universal tool for the monitoring of MDRAB clones.

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页码：5327 / 5331

页数：5

相关论文

共 18 条

[1] Acinetobacter spp, as nosocomial pathogens: Microbiological, clinical, and epidemiological features [J].

BergogneBerezin, E ;

Towner, KJ .

CLINICAL MICROBIOLOGY REVIEWS, 1996, 9 (02) :148-+

[2] PCR-based DNA fingerprinting (REP-PCR, AP-PCR) and pulsed-field gel electrophoresis characterization of a nosocomial outbreak caused by imipenem- and meropenem-resistant Acinetobacter baumannii [J].

Bou, G ;

Cerveró, G ;

Domínguez, MA ;

Quereda, C ;

Martínez-Beltrán, J .

CLINICAL MICROBIOLOGY AND INFECTION, 2000, 6 (12) :635-643

[3] Comparison of outbreak and nonoutbreak Acinetobacter baumannii strains by genotypic and phenotypic methods [J].

Dijkshoorn, L ;

Aucken, H ;

GernerSmidt, P ;

Janssen, P ;

Kaufmann, ME ;

Garaizar, J ;

Ursing, J ;

Pitt, TL .

JOURNAL OF CLINICAL MICROBIOLOGY, 1996, 34 (06) :1519-1525

[4] Emerging importance of multidrug-resistant Acinetobacter species and Stenotrophomonas maltophilia as pathogens in seriously ill patients:: Geographic patterns, epidemiological features, and trends in the SENTRY Antimicrobial Surveillance Program (1997-1999) [J].

Gales, AC ;

Jones, RN ;

Forward, KR ;

Liñares, J ;

Sader, HS ;

Verhoef, J .

CLINICAL INFECTIOUS DISEASES, 2001, 32 :S104-S113

[5]

Hall T.A., 1999, NUCL ACIDS S SER, V41, P95, DOI DOI 10.1021/BK-1999-0734.CH008

[6] Selection of topoisomerase mutations and overexpression of adeB mRNA transcripts during an outbreak of Acinetobacter baumannii [J].

Higgins, PG ;

Wisplinghoff, H ;

Stefanik, D ;

Seifert, H .

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2004, 54 (04) :821-823

[7] Repetitive-DNA-element PCR fingerprinting and antibiotic resistance of pan-European multi-resistant Acinetobacter baumannii clone III strains [J].

Huys, G ;

Cnockaert, M ;

Nemec, A ;

Dijkshoorn, L ;

Brisse, S ;

Vaneechoutte, M ;

Swings, J .

JOURNAL OF MEDICAL MICROBIOLOGY, 2005, 54 (09) :851-856

[8] Distribution of tetracycline resistance genes in genotypically related and unrelated multiresistant Acinetobacter baumannii strains from different European hospitals [J].

Huys, G ;

Cnockaert, M ;

Vaneechoutte, M ;

Woodford, N ;

Nemec, A ;

Dijkshoorn, L ;

Swings, J .

RESEARCH IN MICROBIOLOGY, 2005, 156 (03) :348-355

[9] Surveillance for antimicrobial susceptibility among clinical isolates of Pseudomonas aeruginosa and Acinetobacter baumannii from hospitalized patients in the United States, 1998 to 2001 [J].

Karlowsky, JA ;

Draghi, DC ;

Jones, ME ;

Thornsberry, C ;

Friedland, IR ;

Sahm, DF .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2003, 47 (05) :1681-1688

[10] Resistance-nodulation-cell division-type efflux pump involved in aminoglycoside resistance in Acinetobacter baumannii strain BM4454 [J].

Magnet, S ;

Courvalin, P ;

Lambert, T .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2001, 45 (12) :3375-3380