Keratinocyte growth factor-2 accelerates wound healing in incisional wounds

Background. Keratinocyte growth factor-2 (KGF-2) also described as fibroblast growth factor-10 (FGF-10) is a newly identified member of the fibroblast growth factor family. KGF-2 is 96% identical to the recently identified rat FGF-10 and specifically stimulates growth of normal human epidermal keratinocytes, The present study was undertaken to examine the effects of topically applied KGF-2 in an incisional wound healing model, KGF-2 treatment resulted in an improvement in incisional wound healing as characterized by rue increase in breaking strength, collagen content, and epidermal thickness. Methods. KGF-2 was topically applied to linear incisions made in the dorsal skin of Sprague-Dawley rats. Biomechanical testing was done using an Instron tensiometer for breaking and tensile strength determinations. Wound collagen content was determined using the Sircol collagen assay. Epidermal thickness measurements were conducted using Masson's trichromestained sections of the wound. Results. A single topical application of KGF-2 at the time of wounding resulted in an increase in wound breaking and tensile strength at Day 5 after wounding. Breaking strength of KGF-2-treated wounds was significantly higher compared with the buffer control (1 mu g, 222.1 +/- 13.5 g, P = 0.0007; 4 mu g, 248.7 +/- 15.4 g, P = 0.0001; 10 mu g, 247.2 +/- 21.9 g, P = 0.001; buffer, 141.0 +/- 9.7 g). Epiderm,al thickness and wound collagen content were significantly increased following treatment with KGF-2. Conclusions. Based on our findings, KGF-2 is a potent stimulator of wound healing as demonstrated by increased mechanical strength accompanied by an increase in wound collagen content. KGF-2 could be an important cellular mediator responsible for the initiation and acceleration of wound healing and may enhance the healing of surgical wounds. (C) 1999 Academic Press.