Neurosecretion competence, an independently regulated trait of the neurosecretory cell phenotype

被引:13
作者
Borgonovo, B
Racchetti, G
Malosio, M
Benfante, R
Podini, P
Rosa, P
Meldolesi, J
机构
[1] San Raffaele Sci Inst, DIBIT, Dept Neurosci, I-20132 Milan, Italy
[2] Univ Milan, Dept Pharmacol, B Ceccarelli Ctr, I-20132 Milan, Italy
[3] Univ Milan, Consiglio Nazl Ric, Ctr Mol & Cellular Pharmacol, I-20129 Milan, Italy
关键词
D O I
10.1074/jbc.273.52.34683
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Neurosecretion competence is intended as the ability of neurosecretory cells to express dense and clear vesicles discharged by regulated exocytosis (neurotransmitter release). Such a property, which so far has never been studied independently, is investigated here by a heterotypic cell fusion approach, using a clone of rat pheochromocytoma PC12 cells totally incompetent for neurosecretion that still largely maintains its typical molecular and cellular phenotype. When fused with wild-type partners of various species (rat, human) and specialization (PC12, neuroblastoma SH-SY5Y, HeLa), the defective cells reacquire their competence as revealed by the expression of their secretion-specific proteins. Fused wild-type cells therefore appear able to complement defective cells by providing them with factor(s) inducing the reactivation of their secretory program. The mechanism of action of these factors may consist not in a coordinate unblocking of transcription but in the prevention of a rapid post-transcriptional degradation of the mRNAs for secretion-specific genes.
引用
收藏
页码:34683 / 34686
页数:4
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