Selective immobilization of fusion proteins on poly(hydroxyalkanoate) microbeads

被引：38

作者：

Lee, SJ

Park, JP

Park, TJ

Lee, SY ^{[1
]}

Lee, S ^{[1
]}

Park, JK

机构：

[1] Korea Adv Inst Sci & Technol, Bioproc Engn Res Ctr, Dept Chem & Biomol Engn, Taejon 305701, South Korea

[2] Korea Adv Inst Sci & Technol, Dept Biosyst, Ctr Ultramicrochem Proc Syst, Taejon 305701, South Korea

[3] Korea Adv Inst Sci & Technol, Bioinformat Res Ctr, Taejon 305701, South Korea

来源：

ANALYTICAL CHEMISTRY | 2005年 / 77卷 / 17期

关键词：

D O I：

10.1021/ac0505223

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel fusion protein system employing the substrate-binding domain (SBD) of poly(hydroxyalkanoate) (PHA) depolymerase was developed for the specific immobilization of proteins on PHA microbeads, and was consequently used for immunoassays. The enhanced green fluorescent protein, red fluorescent protein, and severe acute respiratory syndrome coronavirus envelope protein were used as model proteins, and were selectively and functionally immobilized to the PHA microbeads by fusing them to the SBD. Using this PHA microbead system combined with SBD fusion technology, immunoassays could be successfully carried out.

引用

页码：5755 / 5759

页数：5

共 22 条

[1] A microbead-based system for identifying and characterizing RNA-protein interactions by flow cytometry [J].