Characterisation of four species of the genus Kluyveromyces by mitochondrial DNA restriction analysis

被引:26
作者
Belloch, C
Barrio, E
Uruburu, F
Garcia, MD
Querol, A
机构
[1] CSIC,INST AGROQUIM & TECNOL ALIMENTOS,DEPT BIOTECNOL,E-46100 BURJASSOT,VALENCIA,SPAIN
[2] UNIV VALENCIA,COLECC ESPANOLA CULTIVOS TIPO,VALENCIA,SPAIN
[3] UNIV VALENCIA,DEPT GENET,VALENCIA,SPAIN
关键词
Kluyveromyces dobzhanskii; lactis; marxianus and thermotolerans; mtDNA restriction analysis; taxonomy;
D O I
10.1016/S0723-2020(97)80008-2
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Tn the present work, we determine the relationships at the within-species level among strains of Kluyveromyces dobzhanskii, K. lactis, K. marxianus, and K. thermotolerans, through the restriction analysis of their mtDNAs. The three first species showed a high level of intraspecific mtDNA divergence, this polymorphism is correlated to the varieties or species defined according to the original taxonomy of the genus, which is in concordance with that shown by other phenotypic or genotypic markers codified for by the nuclear genome. In these species, the analysis of the relationships among strains based on mtDNA restriction data agrees with previous classifications based on morphological traits and physiological abilities. On the contrary, K. thermotolerans showed a reduced lever of intraspecific divergence compared to the other three, which indicates that this species either is a more recent species, has suffered a reduction of polymorphism due to the action of evolutionary forces, or presents a lower mtDNA substitution rate.
引用
收藏
页码:397 / 408
页数:12
相关论文
共 36 条
[1]  
Barnett J.A., 1990, YEASTS CHARACTERISTI, DOI DOI 10.1046/J.1525-1470.2001.1862020A.X
[2]   Phylogenetic relationships among members of the ascomycetous yeast genera Brettanomyces, Debaryomyces, Dekkem, and Kluyveromyces deduced by small-subunit rRNA gene sequences [J].
Cai, JP ;
Roberts, IN ;
Collins, MD .
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1996, 46 (02) :542-549
[3]  
CORIA OR, 1990, ANTON LEEUW INT J G, V58, P227, DOI 10.1007/BF00399333
[4]  
EXCOFFIER L, 1992, GENETICS, V131, P479
[5]   DEOXYRIBONUCLEIC-ACID BASE SEQUENCE RELATEDNESS AMONG MEMBERS OF THE YEAST GENUS KLUYVEROMYCES [J].
FUSON, GB ;
PRESLEY, HL ;
PHAFF, HJ .
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1987, 37 (04) :371-379
[6]   ORIGIN AND EVOLUTION OF MITOCHONDRIAL-DNA [J].
GRAY, MW .
ANNUAL REVIEW OF CELL BIOLOGY, 1989, 5 :25-50
[7]  
GROSSMAN LT, 1985, GENE MANIPULATIONS F, P60
[8]   Characterization of wine yeast strains of the Saccharomyces genus on the basis of molecular markers: Relationships between genetic distance and geographic or ecological origin [J].
Guillamon, JM ;
Barrio, E ;
Querol, A .
SYSTEMATIC AND APPLIED MICROBIOLOGY, 1996, 19 (01) :122-132
[9]  
Guillamon JM, 1997, FEMS MICROBIOL LETT, V147, P267, DOI 10.1016/S0378-1097(96)00537-X
[10]   Detection of Dekkera-Brettanomyces strains in sherry by a nested PCR method [J].
Ibeas, JI ;
Lozano, I ;
Perdigones, F ;
Jimenez, J .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1996, 62 (03) :998-1003