Protein production and purification

被引：598

作者：

Graslund, Susanne ^{[2
]}

Nordlund, Paer ^{[2
]}

Weigelt, Johan ^{[2
]}

Bray, James ^{[3
]}

Hallberg, B. Martin

Gileadi, Opher ^{[3
]}

Knapp, Stefan ^{[3
]}

Oppermann, Udo ^{[3
]}

Arrowsmith, Cheryl ^{[1
]}

Hui, Raymond ^{[1
]}

Ming, Jinrong ^{[1
]}

dhe-Paganon, Sirano ^{[1
]}

Park, Hee-won ^{[1
]}

Savchenko, Alexei ^{[1
]}

Yee, Adelinda ^{[1
]}

Edwards, Aled ^{[1
]}

Vincentelli, Renaud ^{[4
]}

Cambillau, Christian ^{[4
]}

Kim, Rosalind ^{[5
,6
]}

Kim, Sung-Hou ^{[5
,6
]}

Rao, Zihe ^{[7
]}

Shi, Yunyu ^{[8
]}

Terwilliger, Thomas C. ^{[9
]}

Kim, Chang-Yub ^{[9
]}

Hung, Li-Wei ^{[9
]}

Waldo, Geoffrey S. ^{[9
]}

Peleg, Yoav ^{[10
]}

Albeck, Shira ^{[10
]}

Unger, Tamar ^{[10
]}

Dym, Orly ^{[10
]}

Prilusky, Jaime ^{[10
]}

Sussman, Joel L. ^{[10
]}

Stevens, Ray C. ^{[11
]}

Lesley, Scott A. ^{[11
,12
]}

Wilson, Ian A. ^{[11
,12
]}

Joachimiak, Andrzej ^{[13
]}

Collart, Frank ^{[13
]}

Dementieva, Irina ^{[13
]}

Donnelly, Mark I. ^{[13
]}

Eschenfeldt, William H. ^{[13
]}

Kim, Youngchang ^{[13
]}

Stols, Lucy ^{[13
]}

Wu, Ruying ^{[13
]}

Zhou, Min ^{[13
]}

Burley, Stephen K. ^{[14
]}

Emtage, J. Spencer ^{[14
]}

Sauder, J. Michael ^{[14
]}

Thompson, Devon ^{[14
]}

Bain, Kevin ^{[14
]}

Luz, John ^{[14
]}

机构：

[1] Univ Toronto, Toronto, ON M5G 1L6, Canada

[2] Karolinska Inst, S-17177 Stockholm, Sweden

[3] Univ Oxford, Oxford OX3 7DQ, England

[4] CNRS, F-13288 Marseille 09, France

[5] Univ Calif Berkeley, Lawrence Berkeley Lab, Berkeley, CA 94720 USA

[6] Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA

[7] Tsinghua Univ, Beijing 100084, Peoples R China

[8] Univ Sci & Technol China, Hefei 230027, Peoples R China

[9] Los Alamos Natl Lab, Los Alamos, NM 87507 USA

[10] Weizmann Inst Sci, IL-76100 Rehovot, Israel

[11] Scripps Res Inst, La Jolla, CA 92037 USA

[12] Novartis Res Fdn, Genom Inst, San Diego, CA 92121 USA

[13] Argonne Natl Lab, Biosci Div, Argonne, IL 60439 USA

[14] SGX Pharmaceut Inc, San Diego, CA 92121 USA

[15] Albert Einstein Coll Med, Dept Biochem, Bronx, NY 10461 USA

[16] Brookhaven Natl Lab, Dept Biol, Upton, NY 11973 USA

[17] Case Western Reserve Univ, Cleveland, OH 44016 USA

[18] Univ Calif San Francisco, Dept Biopharmaceut Sci, San Francisco, CA 94143 USA

[19] Univ Calif San Francisco, Dept Pharmaceut Chem, San Francisco, CA 94143 USA

[20] Rutgers State Univ, Ctr Adv Biotechnol & Med, Piscataway, NJ 08854 USA

[21] Columbia Univ, Dept Biol Sci, New York, NY 10027 USA

[22] Wellcome Trust Ctr Human Genet, Div Struct Biol, Oxford OX3 7BN, England

[23] Max Delbruck Ctr Mol Med MDC, D-13092 Berlin, Germany

[24] RIKEN, Yokohama Inst, Genom Sci Ctr, Prot Res Grp, Yokohama, Kanagawa 2300045, Japan

来源：

NATURE METHODS | 2008年 / 5卷 / 02期

基金：

英国医学研究理事会; 英国惠康基金;

关键词：

HIGH-LEVEL EXPRESSION; STRUCTURAL GENOMICS; ESCHERICHIA-COLI; CRYSTAL-STRUCTURE; RECOMBINANT PROTEINS; BINDING-PROTEIN; FUSION PROTEINS; INSECT CELLS; SOLUBILITY; STRATEGIES;

D O I：

10.1038/nmeth.f.202

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In selecting a method to produce a recombinant protein, a researcher is faced with a bewildering array of choices as to where to start. To facilitate decision-making, we describe a consensus 'what to try first' strategy based on our collective analysis of the expression and purification of over 10,000 different proteins. This review presents methods that could be applied at the outset of any project, a prioritized list of alternate strategies and a list of pitfalls that trip many new investigators.

引用

页码：135 / 146

页数：12

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