Mesenchymal stem cell transplantation to promote bone healing

被引：45

作者：

Gao, Chan ^{[1
,2
]}

Seuntjens, Jan ^{[4
,5
]}

Kaufman, Gabriel N. ^{[1
,6
,7
]}

Tran-Khanh, Nicolas ^{[8
]}

Butler, Alison ^{[1
,9
]}

Li, Ailian ^{[1
]}

Wang, Huifen ^{[1
]}

Buschmann, Michael D. ^{[8
]}

Harvey, Edward J. ^{[1
,3
]}

Henderson, Janet E. ^{[1
,2
,3
]}

机构：

[1] JTN Wong Lab Bone Tissue Engn, Innovat Ctr, Montreal, PQ H3A 1A4, Canada

[2] McGill Univ, Dept Med, Res Inst, Ctr Hlth, Montreal, PQ H3H 2R9, Canada

[3] McGill Univ, Dept Surg, Res Inst, Ctr Hlth, Montreal, PQ H3H 2R9, Canada

[4] McGill Univ, Dept Oncol, Res Inst, Ctr Hlth, Montreal, PQ H3H 2R9, Canada

[5] McGill Univ, Med Phys Unit, Res Inst, Ctr Hlth, Montreal, PQ H3H 2R9, Canada

[6] Dept Microbiol, Montreal, PQ H3A 2B4, Canada

[7] Dept Immunol, Montreal, PQ H3A 2B4, Canada

[8] Ecole Polytech, Dept Chem Engn, Montreal, PQ H3T 1J4, Canada

[9] British Columbia Inst Technol, Burnaby, BC V5G 3H2, Canada

来源：

JOURNAL OF ORTHOPAEDIC RESEARCH | 2012年 / 30卷 / 08期

基金：

加拿大健康研究院;

关键词：

MSC transplant; conditioning irradiation; osteopenic mouse; intra-femoral implant; micro CT; FUTURE;

D O I：

10.1002/jor.22028

中图分类号：

R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学（修复外科学）];

学科分类号：

100224 [整形外科学];

摘要：

An overall decline in the availability of osteogenic precursor cells and growth factors in the bone marrow microenvironment have been associated with impaired bone formation and osteopenia in humans. The objective of the current study was to determine if transplantation of mesenchymal stromal cells (MSC) from a healthy, young donor mouse into an osteopenic recipient mouse could enhance osseointegration of a femoral implant. MSC harvested from normal young adult mice differentiated into bone forming osteoblasts when cultured on implant grade titanium surfaces ex vivo and promoted bone formation around titanium-coated rods implanted in the femoral canal of osteopenic recipient mice. Micro computed tomographic imaging and histological analyses showed more, better quality, bone in the femur that received the MSC transplant compared with the contra-lateral control femur that received carrier alone. These results provide pre-clinical evidence that MSC transplantation promotes peri-implant bone regeneration and suggest the approach could be used in a clinical setting to enhance bone regeneration and healing in patients with poor quality bone. (C) 2012 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 30:11831189, 2012

引用

页码：1183 / 1189

页数：7

共 22 条

[1]

Prospects for osteoprogenitor stem cells in fracture repair and osteoporosis [J].