Simulated microgravity using a rotary cell culture system promotes chondrogenesis of human adipose-derived mesenchymal stem cells via the p38 MAPK pathway

被引：96

作者：

Yu, Bo ^{[1
,2
]}

Yu, Degang ^{[1
]}

Cao, Lei ^{[1
]}

Zhao, Xin ^{[1
]}

Long, Teng ^{[1
]}

Liu, Guangwang ^{[1
]}

Tang, Tingting ^{[1
]}

Zhu, Zhenan ^{[1
]}

机构：

[1] Shanghai Jiao Tong Univ, Sch Med, Shanghai Peoples Hosp 9, Dept Orthopaed,Shanghai Key Lab Orthopaed Implant, Shanghai 200011, Peoples R China

[2] Shandong Univ Tradit Chinese Med, Affiliated Hosp, Dept Orthopaed, Jinan 250014, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2011年 / 414卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Microgravity; p38; Rotary cell culture system; Adipose derived stem cells; ROTATING-WALL VESSEL; PROTEIN-KINASE; MODELED MICROGRAVITY; ARTICULAR-CARTILAGE; PROGENITOR CELLS; TISSUE-GROWTH; STROMAL CELLS; CROSS-TALK; DIFFERENTIATION; BONE;

D O I：

10.1016/j.bbrc.2011.09.103

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Mesenchymal stem cells (MSCs) are multi-potent, and the chondrogenesis of MSCs is affected by mechanical stimulation. The aim of this study was to investigate, using a rotary cell culture system (RCCS) bioreactor, the effects of microgravity on the chondrogenic differentiation of human adipose-derived MSCs (ADSCs), which were cultured in pellets with or without the chondrogenic growth factor TGF-beta 1. In addition, we evaluated the role of the p38 MAPK pathway in this process. The real-time PCR and histological results show that microgravity has a synergistic effect on chondrogenesis with TGF-beta 1. The p38 MAPK pathway was activated by TGF-beta 1 alone and was further stimulated by microgravity. Inhibition of p38 activity with 5B203580 suppressed chondrocyte-specific gene expression and matrix production. These findings suggest that the p38 MAPK signal acts as an essential mediator in the microgravity-induced chondrogenesis of ADSCs. (C) 2011 Elsevier Inc. All rights reserved.

引用

页码：412 / 418

页数：7

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