Use of design of experiments to optimize high-throughput semipreparative LC and LC/MS methods

被引：17

作者：

Cole, DC ^{[1
]}

Pagano, N ^{[1
]}

Kelly, MF ^{[1
]}

Ellingboe, J ^{[1
]}

机构：

[1] Wyeth Ayerst Res, Chem & Screening Sci, Pearl River, NY 10965 USA

来源：

JOURNAL OF COMBINATORIAL CHEMISTRY | 2004年 / 6卷 / 01期

关键词：

D O I：

10.1021/cc034013v

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

This paper describes an investigation into decreasing the run time for high-throughput semipreparative RP-HPLC methods without compromising the resolution. Experimental design was used to devise a small set of experiments in which factors, including solvent flow rate, solvent/column temperature, at-column dilution, and run time were varied systematically. The results were analyzed by means of multiple regression and partial least squares to generate a model relating the factors to the results, showing which factors are important. The model was then used to determine the optimal conditions.

引用

页码：78 / 82

页数：5