Osteogenic proliferation and differentiation of canine bone marrow and adipose tissue derived mesenchymal stromal cells and the influence of hypoxia

被引:52
作者
Chung, Dai-Jung [2 ]
Hayashi, Kei [2 ]
Toupadakis, Chrisoula A. [1 ]
Wong, Alice [1 ]
Yellowley, Clare E. [1 ]
机构
[1] Univ Calif Davis, Dept Anat Physiol & Cell Biol, Davis, CA 95616 USA
[2] Univ Calif Davis, Dept Surg & Radiol Sci, Davis, CA 95616 USA
关键词
Canine mesenchymal stromal cell; Bone marrow; Adipose tissue; Proliferation; Osteogenic differentiation; Hypoxia; UMBILICAL-CORD BLOOD; REDUCED OXYGEN-TENSION; STEM-CELLS; IN-VITRO; EXPRESSION; CHONDROGENESIS; DOGS; REGENERATION; PERIOSTEUM; FRACTURES;
D O I
10.1016/j.rvsc.2010.10.012
中图分类号
S85 [动物医学(兽医学)];
学科分类号
090604 [动物药学];
摘要
The aim of this study was to compare the osteogenic and proliferative potential of canine mesenchymal stromal cells (cMSCs) derived from bone marrow (BM-cMSCs) and adipose tissue (AT-cMSCs). Proliferation potential was determined under varying oxygen tensions (1%, 5%, and 21% O-2). Effects of reduced oxygen levels on the osteogenic differentiation of AT-cMSCs were also investigated. AT-cMSCs proliferated at a significantly faster rate than BM-cMSCs, although both cell types showed robust osteogenic differentiation. Culture in 5% and 1% O-2 impaired proliferation in cMSC from both sources and osteogenic differentiation in AT-cMSCs. Our data suggests that AT-cMSCs might be more suitable for use in a clinical situation, where large cell numbers are required for bone repair, due to their rapid proliferation combined with robust osteogenic potential. Our data also suggests that the inhibitory effects of hypoxia on both cell proliferation and differentiation should be considered when using MSCs in a potentially hypoxic environment such as a fracture site. (C) 2010 Elsevier Ltd. All rights reserved.
引用
收藏
页码:66 / 75
页数:10
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