Structure, chain conformation and antitumor activity of a novel polysaccharide from Lentinus edodes

被引：231

作者：

Zhang Yu ^{[2
]}

Gu Ming ^{[2
]}

Wang Kaiping ^{[1
]}

Chen Zhixiang ^{[1
]}

Dai Liquan ^{[1
]}

Liu Jingyu ^{[1
]}

Zeng Fang ^{[1
]}

机构：

[1] Huazhong Univ Sci & Technol, Pharmaceut Sch, Tongji Med Coll, Hubei Key Lab Nat Med Chem & Resource Evaluat, Wuhan 430030, Peoples R China

[2] Huazhong Univ Sci & Technol, Union Hosp, Wuhan 430030, Peoples R China

来源：

FITOTERAPIA | 2010年 / 81卷 / 08期

关键词：

Lentinus edodes; Polysaccharide; Chemical structure; Chain conformation; Antitumor activity; ANTI-TUMOR POLYSACCHARIDE; CHLORELLA-PYRENOIDOSA; MOLECULAR-WEIGHT; EDIBLE-MUSHROOM; SOLID-STATE; IN-VITRO; SCHIZOPHYLLAN; TRIPLE; PURIFICATION; SPECTROSCOPY;

D O I：

10.1016/j.fitote.2010.07.019

中图分类号：

R914 [药物化学];

学科分类号：

100705 [微生物与生化药学];

摘要：

A water-soluble polysaccharide LT1 was isolated from the basidiocarps of Lentinus edodes by hot water extraction and ethanol precipitations, further purified by gel chromatography. The Mw of LT1 was estimated to be 642 kDa by using HPGPC. Chemical and spectroscopic studies illustrated that LT1 has a backbone chain composed of 1 -> 4-linked and 1 -> 3-linked glucopyranosyl residues and has branches of single glucosyl stubs at C-6 of beta-(1 -> 4)-linked glucopyranosyl. AFM and Congo-red test revealed that LT1 existed as triple helix chain in 0.10 M NaOH solution or distilled water. Our studies showed that LT1 presented significant antitumor bioactivities on Sarcoma180 solid tumor cell implanted in BALB/c mice, which implies that LT1 could be potentially applied as a natural antitumor drug. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：1163 / 1170

页数：8

共 36 条

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NMR-SPECTROSCOPY IN THE STRUCTURAL ELUCIDATION OF OLIGOSACCHARIDES AND GLYCOSIDES [J].