Genetic analysis of the DBC2 gene in gastric cancer

被引：29

作者：

Cho, Yong Gu ^{[1
]}

Choi, Byung Joon ^{[2
]}

Kim, Chang Jae ^{[1
]}

Song, Jae Hwi ^{[1
]}

Zhang, Cao ^{[1
]}

Nam, Suk Woo ^{[1
]}

Lee, Jung Young ^{[1
]}

Park, Won Sang ^{[1
]}

机构：

[1] Catholic Univ Korea, Coll Med, Dept Pathol, Seoul 137701, South Korea

[2] Catholic Univ Korea, Coll Med, Dept Pediat, Seoul, South Korea

来源：

ACTA ONCOLOGICA | 2008年 / 47卷 / 03期

关键词：

D O I：

10.1080/02841860701644094

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 [肿瘤学];

摘要：

The DBC2 (Deleted in breast cancer, RhoBTB2) has been identified as a tumor suppressor gene that has growth inhibitory function. To investigate whether genetic alterations of the DBC2 gene are involved in the development of gastric cancer, we analyzed mutations and allelic loss in the DBC2 gene in 95 primary gastric cancers by PCR-SSCP, sequencing and LOH analysis. In the mutational analysis, we found one missense somatic mutation (CGG -> TGG, R275W) in the BTB/POZ domain of the gene in a patient with advanced gastric cancer and lymph node metastasis. In addition, we found one known polymorphism and three novel polymorphisms in the coding region of DBC2, which showed an amino acid change, and was detected in both the cancer cells and corresponding normal cells. On LOH analysis, 62 cases were heterozygous for at least one marker and 18 cases (29.0%) showed allelic loss at these markers. In conclusion, the mutations and allelic loss in the DBC2 gene are uncommon in gastric cancers in Korean patients. Further studies to identify the target gene at 8q21 responsible for the development of gastric cancer should be explored.

引用

页码：366 / 371

页数：6

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