Analysis of theaflavins in biological fluids using liquid chromatography-electrospray mass spectrometry

被引：135

作者：

Mulder, TPJ

van Platerink, CJ

Schuyl, PJW

van Amelsvoort, JMM

机构：

[1] Unilever Res Labs Vlaardingen, Unilever Hlth Inst, NL-3130 AC Vlaardingen, Netherlands

[2] Unilever Res Labs Vlaardingen, Cent Analyt Sci, Vlaardingen, Netherlands

来源：

JOURNAL OF CHROMATOGRAPHY B | 2001年 / 760卷 / 02期

关键词：

theaflavins;

D O I：

10.1016/S0378-4347(01)00285-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A HPLC-MS procedure for the sensitive and specific analysis of the black tea flavonoid theaflavin in human plasma and urine was developed. Levels were measured after enzymatic deconjugation, extraction into ethyl acetate, and separation by HPLC, using tandem mass spectrometry as a detecting system. Two healthy volunteers consumed 700 mg theaflavins, equivalent to about 30 cups of black tea. The maximum concentration detected in blood plasma was 1.0 mug l(-1) in a sample collected after 2 h. The concentration in urine also peaked after 2 h at 4.2 mug l(-1). Hence, only minute amounts of theaflavins can be detected in plasma and urine samples of healthy volunteers after ingestion. (C) 2001 Elsevier Science BV All rights reserved.

引用

页码：271 / 279

页数：9

共 31 条

[1] Determination of selected phytochemicals by reversed-phase high-performance liquid chromatography combined with ultraviolet and mass spectrometric detection [J].