Cloning and characterization of KLHL5, a novel human gene encoding a kelch-related protein with a BTB domain

被引：8

作者：

Wang, S ^{[1
]}

Zhou, ZX ^{[1
]}

Ying, K ^{[1
]}

Tang, R ^{[1
]}

Huang, Y ^{[1
]}

Wu, CQ ^{[1
]}

Xie, Y ^{[1
]}

Mao, YM ^{[1
]}

机构：

[1] Fudan Univ, Sch Life Sci, Inst Genet, State Key Lab Genet Engn, Shanghai 200433, Peoples R China

来源：

BIOCHEMICAL GENETICS | 2001年 / 39卷 / 7-8期

关键词：

human KLHL5 gene; kelch; BTB domain; BTB/kelch protein; actin binding;

D O I：

10.1023/A:1010203114697

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Most kelch family proteins contain two conserved domains, the BTB domain and the kelch repeat domain. Here we describe the cloning and characterization of a novel human KLHL5 gene. The 3488 bp cDNA encodes a kelch family protein homologous to the Drosophila kelch protein. It also contains the two conserved domains. Northern blot analysis reveals a single transcript. It is abundantly expressed in ovary, adrenal gland, and thyroid and less abundantly expressed in trachea, prostate, testis, lymph node, and spinal cord. KLHL5 was mapped to 4p13-4p15.1.

引用

页码：227 / 238

页数：12

共 23 条

[1] The kelch repeat superfamily of proteins: propellers of cell function [J].

Adams, J ;

Kelso, R ;

Cooley, L .

TRENDS IN CELL BIOLOGY, 2000, 10 (01) :17-24

[2] The gene encoding gigaxonin, a new member of the cytoskeletal BTB/kelch repeat family, is mutated in giant axonal neuropathy [J].

Bomont, P ;

Cavalier, L ;

Blondeau, F ;

Hamida, CB ;

Belal, S ;

Tazir, M ;

Demir, E ;

Topaloglu, H ;

Korinthenberg, R ;

Tüysüz, B ;

Landrieu, P ;

Hentati, F ;

Koenig, M .

NATURE GENETICS, 2000, 26 (03) :370-374

[3] DROSOPHILA KELCH MOTIF IS DERIVED FROM A COMMON ENZYME FOLD [J].

BORK, P ;

DOOLITTLE, RF .

JOURNAL OF MOLECULAR BIOLOGY, 1994, 236 (05) :1277-1282

[4] Drosophila ring canal growth requires Src and Tec kinases [J].

Cooley, L .

CELL, 1998, 93 (06) :913-915

[5]

DIBELLO PR, 1991, GENETICS, V129, P385

[6] THE COMPLETE DNA-SEQUENCE OF VACCINIA VIRUS [J].

GOEBEL, SJ ;

JOHNSON, GP ;

PERKUS, ME ;

DAVIS, SW ;

WINSLOW, JP ;

PAOLETTI, E .

VIROLOGY, 1990, 179 (01) :247-266

[7] Functional cooperation between the microtubule and actin cytoskeletons [J].

Goode, BL ;

Drubin, DG ;

Barnes, G .

CURRENT OPINION IN CELL BIOLOGY, 2000, 12 (01) :63-71

[8]

HARRISON SD, 1990, EMBO, V9, P799

[9]

Hernandez MC, 1997, J NEUROSCI, V17, P3038

[10]

JOESPHINE CA, 1998, EMBO, V17, P4964

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