Extremely rapid extraction of DNA from bacteria and yeasts

被引：354

作者：

Cheng, HR

Jiang, N ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Microbiol, Ctr Microbial Biotechnol, Beijing 100080, Peoples R China

[2] Chinese Acad Sci, Grad Sch, Beijing 100080, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2006年 / 28卷 / 01期

关键词：

bacteria; DNA extraction; yeast;

D O I：

10.1007/s10529-005-4688-z

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A very simple and rapid method for extracting genomic DNA from Gram-negative bacteria, Gram-positive bacteria and yeasts is presented. In this method, bacteria or yeasts are lysed directly by phenol and the supernatant is extracted with chloroform to remove traces of phenol. The supernatant contains DNA that is suitable for molecular analyses, such as PCR, restriction enzyme digestion and genomic library construction. This method is reproducible and simple for the routine DNA extraction from bacteria and yeasts.

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收藏

页码：55 / 59

页数：5

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