Indirect immunoperoxidase technique for the diagnosis of cold-water disease in cultured ayu, Plecoglossus altivelis

Indirect immunoperoxidase technique (HPT) was developed for the diagnosis of cold-water disease in cultured ayu (Plecoglossus altivelis). Cytophaga psychrophila cells were clearly distinguished from Pseudomonas sp. cells, the causative bacterium of bacterial hemorrhagic ascites of cultured ayu, in the cross reaction test. The gills, spleens and skins from naturally infected fish were examined for C. psychrophila by LIFT and cultivation with agar plates. The results suggested that IIPT was a quick and reliable method for the diagnosis of cold-water disease.