Utility of flow cytometric analysis of mast cells in the diagnosis and classification of adult mastocytosis

被引：107

作者：

Escribano, L

Díaz-Agustín, B

Bellas, C

Navalón, R

Nuñez, R

Sperr, WR

Schernthaner, GH

Valent, P

Orfao, A

机构：

[1] Hosp Ramon y Cajal, Mast Cell Unit, Serv Hematol, E-28034 Madrid, Spain

[2] Hosp Ramon y Cajal, Serv Anat Patol, E-28034 Madrid, Spain

[3] Univ Vienna, Div Haematol & Haemostaseol, Dept Internal Med 1, A-1010 Vienna, Austria

[4] Univ Salamanca, Dept Med, Serv Gen Citometr, E-37008 Salamanca, Spain

[5] Univ Salamanca, Ctr Invest Canc, E-37008 Salamanca, Spain

来源：

LEUKEMIA RESEARCH | 2001年 / 25卷 / 07期

基金：

奥地利科学基金会;

关键词：

mast cells; mastocytosis; diagnosis; immunophenotype; flow cytometry; CD2; CD25;

D O I：

10.1016/S0145-2126(01)00050-9

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The diagnosis of bone marrow (BM) involvement in mastocytosis has mainly been based on conventional histology. Nevertheless, in recent years, three major methodological advances have been made: the measurement of serum tryptase levels, the immunohistochemical assessment of mast cell (MC) tryptase, and the immunophenotypical characterization of BMMC using flow cytometry (FCM). The most characteristic immunophenotypic feature in mastocytosis is the coexpression of CD2 and CD25 antigens, which are never present in normal BMMC and constitute a phenotypic hallmark of BMMC in adult mastocytosis. Such observations would support the need to include the immunophenotypic analysis of MC in the diagnosis of mastocytosis. (C) 2001 Elsevier Science Ltd. All rights reserved.

引用

页码：563 / 570

页数：8

共 66 条

[1] Comparative immunophenotypic analysis of human mast cells, blood basophils and monocytes [J].

Agis, H ;

Fureder, W ;

Bankl, HC ;

Kundi, M ;

Sperr, WR ;

Willheim, M ;

BoltzNitulescu, G ;

Butterfield, JH ;

Kishi, K ;

Lechner, K ;

Valent, P .

IMMUNOLOGY, 1996, 87 (04) :535-543

[2]

AGUSTIN BD, 1999, BRIT J HAEMATOL, V106, P400

[3] The biology of stem cell factor and its receptor C-kit [J].

Ashman, LK .

INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 1999, 31 (10) :1037-1051

[4] SYSTEMIC MASTOCYTOSIS [J].

AUSTEN, KF .

NEW ENGLAND JOURNAL OF MEDICINE, 1992, 326 (09) :639-640

[5]

BRIZZI MF, 1994, ONCOGENE, V9, P1583

[6] SYSTEMIC MASTOCYTOSIS - EXTRACUTANEOUS MANIFESTATIONS [J].

BRUNNING, RD ;

MCKENNA, RW ;

ROSAI, J ;

PARKIN, JL ;

RISDALL, R .

AMERICAN JOURNAL OF SURGICAL PATHOLOGY, 1983, 7 (05) :425-438

[7]

Brunning RD, 1994, TUMORS BONE MARROW, P409

[8]

Cerveró C, 1999, AM J HEMATOL, V60, P191, DOI 10.1002/(SICI)1096-8652(199903)60:3<191::AID-AJH4>3.0.CO

[9]

2-Y

[10]

DASTYCH J, 1994, J IMMUNOL, V152, P213

← 1 2 3 4 5 6 7 →