Automatic high-performance liquid chromatographic method for the determination of diarrhetic shellfish poison

被引：13

作者：

Hummert, C

Shen, JL

Luckas, B

机构：

来源：

JOURNAL OF CHROMATOGRAPHY A | 1996年 / 729卷 / 1-2期

关键词：

shellfish; column switching; okadaic acid; dinophysistoxin-1; phycotoxins; toxins;

D O I：

10.1016/0021-9673(95)01261-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid HPLC method with fluorescence detection for the determination of okadaic acid (OA) and dinophysistoxin-1 (DTX-1) in mussels and mussel products is presented. For fluorescence labeling of OA and DTX-1, 9-anthryldiazomethane (ADAM) is used. HPLC with a column-switching system is proposed to avoid time-consuming clean-up procedures after derivatization of sample extracts with ADAM. The column-switching system as well as the chromatographic conditions and detection are described.

引用

收藏

页码：387 / 392

页数：6

相关论文

共 13 条

[1]

[Anonymous], 1984, DINOFLAGELLATES

[2] IDENTIFICATION OF OKADAIC ACID FROM A CARIBBEAN DINOFLAGELLATE, PROROCENTRUM-CONCAVUM [J].

DICKEY, RW ;

BOBZIN, SC ;

FAULKNER, DJ ;

BENCSATH, FA ;

ANDRZEJEWSKI, D .

TOXICON, 1990, 28 (04) :371-377

[3] SEPARATION OF THE 9-ANTHRYLDIAZOMETHANE DERIVATES OF FATTY-ACIDS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ON A FATTY-ACID ANALYSIS COLUMN - APPLICATION TO ALBUMIN-BOUND FATTY-ACID ANALYSIS [J].

GHIGGERI, GM ;

CANDIANO, G ;

DELFINO, G ;

QUEIROLO, C ;

GINEVRI, F ;

PERFUMO, F ;

GUSMANO, R .

JOURNAL OF CHROMATOGRAPHY, 1986, 381 (02) :411-418

[4] Determination of diarrhetic shellfish toxins in various dinoflagellate species [J].

Lee, Jong-Soo ;

Igarashi, Teruo ;

Fraga, Santiago ;

Dahl, Einal ;

Hovgaard, Peter ;

Yasumotol, Takeshi .

JOURNAL OF APPLIED PHYCOLOGY, 1989, 1 (02) :147-152

[5] FLUOROMETRIC-DETERMINATION OF DIARRHETIC SHELLFISH TOXINS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

LEE, JS ;

YANAGI, T ;

KENMA, R ;

YASUMOTO, T .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1987, 51 (03) :877-881

[6]

LEE JS, 1989, BIOACT MOL, V10, P327

[7] ON THE OCCURRENCE AND DETERMINATION OF OKADAIC ACID IN MUSSELS FROM THE GERMAN NORTH-SEA COAST [J].

LUCKAS, B ;

MEIXNER, R .

ZEITSCHRIFT FUR LEBENSMITTEL-UNTERSUCHUNG UND-FORSCHUNG, 1988, 187 (05) :421-424

[8] PHYCOTOXINS IN SEAFOOD - TOXICOLOGICAL AND CHROMATOGRAPHIC ASPECTS [J].

LUCKAS, B .

JOURNAL OF CHROMATOGRAPHY, 1992, 624 (1-2) :439-456

[9]

LUCKAS B, 1994, 15339 BCR EUR EN DIR

[10]

MURATA M, 1982, B JPN SOC SCI FISH, V48, P549