Redirection of biochemical pathways for the enhancement of H2 production by Enterobacter cloacae

被引：66

作者：

Kumar, N ^{[1
]}

Ghosh, A ^{[1
]}

Das, D ^{[1
]}

机构：

[1] Indian Inst Technol, Dept Biotechnol, Kharagpur 721302, W Bengal, India

来源：

BIOTECHNOLOGY LETTERS | 2001年 / 23卷 / 07期

关键词：

alcohol dehydrogenase; butanediol dehydrogenase; Enetrobacter cloacae; H-2; metabolic flux;

D O I：

10.1023/A:1010334803961

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Improvement in H-2 production was achieved through redirection of metabolic pathways by blocking formation of alcohol and some organic acids in Enterobacter cloacae IIT-BT 08. The wild type strain was more susceptible to allyl alcohol (7 mM) and to the combined effect of NaBr and NaBrO3 (40 mM each at pH 5.5) than were double mutants, with defects in both alcohol and organic acid formation pathways, which had higher H-2 yields (3.4 mol mol(-1) glucose) than the wild type strain (2.1 mol mol(-1) glucose).

引用

页码：537 / 541

页数：5

共 11 条

[1] ESCHERICHIA-COLI MUTANTS WITH ALTERED CONTROL OF ALCOHOL-DEHYDROGENASE AND NITRATE REDUCTASE [J].

CLARK, D ;

CRONAN, JE .

JOURNAL OF BACTERIOLOGY, 1980, 141 (01) :177-183

[2] BIOLOGICAL FORMATION OF MOLECULAR HYDROGEN [J].

GRAY, CT ;

GEST, H .

SCIENCE, 1965, 148 (3667) :186-&

[3]

JEFFERY WW, 1984, J BACTERIOL, V160, P687

[4] PHYSIOLOGICAL AND BIOCHEMICAL ROLE OF BUTANEDIOL PATHWAY IN AEROBACTER (ENTEROBACTER) AEROGENES [J].

JOHANSEN, L ;

BRYN, K ;

STORMER, FC .

JOURNAL OF BACTERIOLOGY, 1975, 123 (03) :1124-1130

[5] Enhancement of hydrogen production by Enterobacter cloacae IIT-BT 08 [J].

Kumar, N ;

Das, D .

PROCESS BIOCHEMISTRY, 2000, 35 (06) :589-593

[6] Production and purification of α-amylase from hydrogen producing Enterobacter cloacae IIT-BT 08 [J].

Kumar, N ;

Das, D .

BIOPROCESS ENGINEERING, 2000, 23 (02) :205-208

[7]

KUMAR N, 1999, Patent No. 2000473

[8]

KUMAR N, 1999, P 9 EUR C BIOT 11 15

[9]

LOROWITZ W, 1982, J BACTERIOL, V152, P935

[10] HYDROGEN-PRODUCTION BY IMMOBILIZED CELLS IN THE NOZZLE LOOP BIOREACTOR [J].

SEON, YH ;

LEE, CG ;

PARK, DH ;

HWANG, KY ;

JOE, YI .

BIOTECHNOLOGY LETTERS, 1993, 15 (12) :1275-1280

← 1 2 →