Preparation of cultured myofibers from larval salamander limbs for cellular plasticity studies

被引:4
作者
Kumar, Anoop [1 ]
Brockes, Jeremy P. [1 ]
机构
[1] UCL, Dept Biochem & Mol Biol, London WC1E 6BT, England
基金
英国医学研究理事会;
关键词
D O I
10.1038/nprot.2007.134
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Identification of the mechanisms underlying cellular plasticity in salamander cells is important because these may give pointers to the restricted regenerative ability of mammals. The myofibers from salamanders are remarkable for their ability to undergo cellularization and cell-cycle re-entry during regeneration. Here, we describe a detailed method for the isolation and culture of larval salamander myofibers in numbers suitable for cellular plasticity studies. The basic protocol for isolation and purification of cells can be completed in 4 h.
引用
收藏
页码:939 / 947
页数:9
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