Modulation of neutrophil adhesion to vascular endothelial cells in rat experimental otitis media treated with a macrolide

被引:3
作者
Enomoto, F
Kin, R
Kataoka, T
Yamazaki, Y
Yokoi, H
Fujimori, M
Nagaoka, I
Ichikawa, G
机构
[1] Juntendo Univ, Sch Med, Dept Otorhinolaryngol, Bunkyo Ku, Tokyo 1138421, Japan
[2] Juntendo Univ, Urayasu Hosp, Dept Otorhinolaryngol, Bunkyo Ku, Tokyo 1138421, Japan
[3] Juntendo Univ, Sch Med, Dept Biochem, Bunkyo Ku, Tokyo 1138421, Japan
关键词
endothelial cell; neutrophil; adhesion molecule; L-selectin; Mac-1;
D O I
10.1016/S0385-8146(03)00097-X
中图分类号
R76 [耳鼻咽喉科学];
学科分类号
100213 ;
摘要
Object: We previously reported that erythromycin (EM) affected the adhesion molecules that interact with neutrophils and inhibited neutrophil infiltration in a rat experimental otitis media model. Purpose: The purpose of the present Study was to examine whether EM affects the adhesion of rat neutrophils to endothelial cells. Method: The adhesion of neutrophils to WK-5 endothelial cells was evaluated. One group of rats was given EM for 2 weeks, while the other group received no treatment. The adhesion of peripheral-blood neutrophils and neutrophils accumulated in the middle ear from these two groups were compared. Results: Two-week EM pretreatment inhibited adhesion of neutrophils to WK-5 cells. The adhesion of neutrophils accumulated in the middle ear cavity to WK-5 cells was significantly higher than that of peripheral-blood neutrophils from control rats. Conclusion: The results suggest that EM affects the adhesion of neutrophils to endothelial cells. (C) 2003 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:247 / 251
页数:5
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