The Protein Import Channel in the Outer Mitosomal Membrane of Giardia intestinalis

被引：45

作者：

Dagley, Michael J. ^{[1
,2
]}

Dolezal, Pavel ^{[3
]}

Likic, Vladimir A. ^{[2
]}

Smid, Ondrej ^{[3
]}

Purcell, Anthony W. ^{[1
,2
]}

Buchanan, Susan K. ^{[4
]}

Tachezy, Jan ^{[3
]}

Lithgow, Trevor ^{[1
,5
]}

机构：

[1] Univ Melbourne, Dept Biochem & Mol Biol, Parkville, Vic 3052, Australia

[2] Univ Melbourne, Mol Sci & Biotechnol Inst Bio21, Parkville, Vic 3052, Australia

[3] Charles Univ Prague, Fac Sci, Dept Parasitol, Prague 2, Czech Republic

[4] NIDDK, Mol Biol Lab, US NIH, Bethesda, MD USA

[5] Monash Univ, Dept Biochem & Mol Biol, Clayton, Vic, Australia

来源：

MOLECULAR BIOLOGY AND EVOLUTION | 2009年 / 26卷 / 09期

基金：

澳大利亚研究理事会; 英国医学研究理事会; 美国国家卫生研究院;

关键词：

mitochondria; mitosomes; Giardia intestinalis; protein translocation; evolution; MITOCHONDRIAL-MEMBRANE; MICROSPORIDIAN MITOSOMES; INTERMEMBRANE SPACE; YEAST MITOCHONDRIA; LAMBLIA; MATURATION; RECEPTORS; EVOLUTION; TRYPANOSOMA; TRANSLOCASE;

D O I：

10.1093/molbev/msp117

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The identification of mitosomes in Giardia generated significant debate on the evolutionary origin of these organelles, whether they were highly reduced mitochondria or the product of a unique endosymbiotic event in an amitochondrial organism. As the protein import pathway is a defining characteristic of mitochondria, we sought to discover a TOM (translocase in the outer mitochondrial membrane) complex in Giardia. A Hidden Markov model search of the Giardia genome identified a Tom40 homologous sequence (GiTom40), where Tom40 is the protein translocation channel of the TOM complex. The GiTom40 protein is located in the membrane of mitosomes in a similar to 200-kDa TOM complex. As Tom40 was derived in the development of mitochondria to serve as the protein import channel in the outer membrane, its presence in Giardia evidences the mitochondrial ancestry of mitosomes.

引用

页码：1941 / 1947

页数：7

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