Successful vaccination against Onchocerca ochengi infestation in cattle using live Onchocerca volvulus infective larvae

被引:24
作者
Achukwi, M. D.
Harnett, W.
Enyong, P.
Renz, A.
机构
[1] Wakwa Reg Ctr, Inst Agr Res Dev, Ngaoundere, Cameroon
[2] Univ Strathclyde, Dept Immunol, Glasgow G4 0NR, Lanark, Scotland
[3] IMPM, Med Res Ctr, Kumba, Cameroon
[4] Univ Tubingen, Tierphysiol Inst, D-72076 Tubingen, Germany
关键词
cattle; IgG subclass antibodies; infective larvae; lymphocytes; Onchocerca ochengi; Onchocerca volvulus; recombinant O; volvulus antigens; vaccination;
D O I
10.1111/j.1365-3024.2006.00917.x
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Epidemiological evidence has led to the hypothesis that the concurrent and predominant transmission of Onchocerca ochengi by Simulium damnosum s.l. in sub-Saharan Africa could lead to the protection of humans against onchocerciasis caused by Onchocerca volvulus (zooprophylaxis). To gain support for this hypothesis, we investigated whether exposure to O. volvulus could protect cattle from O. ochengi. Gudali calves were vaccinated with live O. volvulus-infective larvae and subsequently challenged with O. ochengi-infective larvae whilst raised in a fly-proof house. Post-challenge adult parasite and microfilaria development, IgG1 and lgG2 subclass antibodies response to Ov10/Ov11 recombinant Onchocerca antigens, and peripheral blood lymphocyte proliferative responses to O. ochengi crude antigens were studied over a 1-year period. The vaccinated-challenged animals had 83-87% less adult O. ochengi parasites than non-vaccinated-challenged animals. IgG1 and lgG2 antibodies to Ov10/Ov11 recombinant Onchocerca antigens were invoked by non-vaccinated-challenged animals but not by most (80%) of the vaccinated-challenged animals. These findings support the idea of cross-protection (zooprophylaxis) due to inoculation of humans with O. ochengi-infective larvae under natural transmission conditions in endemic areas.
引用
收藏
页码:113 / 116
页数:4
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