Validation of single nucleotide polymorphism quantification in pooled DNA samples with SNaPIT™ -: A glycosylase-mediated methods for polymorphism detection method

被引：5

作者：

Curran, S ^{[1
]}

Hill, L ^{[1
]}

O'Grady, G ^{[1
]}

Turic, D ^{[1
]}

Asherson, P ^{[1
]}

Taylor, E ^{[1
]}

Sham, P ^{[1
]}

Craig, I ^{[1
]}

Vaughan, P ^{[1
]}

机构：

[1] Inst Psychiat, SGDP Res Ctr, London SE5 8AF, England

来源：

MOLECULAR BIOTECHNOLOGY | 2002年 / 22卷 / 03期

关键词：

allele frequency; genotyping; glycosidase; single nucleotide polymorphisms; SNaPIT technology;

D O I：

10.1385/MB:22:3:253

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Association studies using genome scans to identify quantitative trait loci for multifactorial, disorders, with anything approaching reasonable power, have been compromised by the need for a very dense array of genetic markers and large numbers of affected individuals. These requirements impose enormous burdens on the genotyping capacity for most laboratories. DNA pooling,has been proposed as a possible approach to reduce genotyping costs and effort. We report on the application of the SNaPIT(TM) technology to evaluate allele frequencies in pooled DNA samples and conclude that it offers a cost effective, efficient and accurate estimator and provides several advantages over competing technologies in this regard.

引用

页码：253 / 262

页数：10

共 29 条

[1] Association mapping of disease loci, by use of a pooled DNA genomic screen [J].