Cell-surface heparan sulfate is involved in the repulsive guidance activities of Slit2 protein

被引：169

作者：

Hu, HY ^{[1
]}

机构：

[1] Metrohlth Med Ctr, Rammelkamp Ctr Educ & Res, Cleveland, OH 44109 USA

来源：

NATURE NEUROSCIENCE | 2001年 / 4卷 / 07期

关键词：

D O I：

10.1038/89482

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Slit proteins are a family of secreted guidance proteins that can repel neuronal migration and axon growth via interaction with their cellular roundabout receptors (Robos). Here it was shown that Slit2-Robo-1 interactions were enhanced by cell-surface heparan sulfate. Removal of heparan sulfate decreased the affinity of Slit for Robe by about threefold. In addition, removal of cell-surface heparan sulfate by heparinase III abolished the chemorepulsive response to Slit2 normally shown by both the migrating neurons and growing axons. These results indicate essential roles for cell-surface heparan sulfate in the repulsive activities of Slit2.

引用

页码：695 / 701

页数：7

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