Cell-surface heparan sulfate is involved in the repulsive guidance activities of Slit2 protein

被引：169

作者：

Hu, HY ^{[1
]}

机构：

[1] Metrohlth Med Ctr, Rammelkamp Ctr Educ & Res, Cleveland, OH 44109 USA

来源：

NATURE NEUROSCIENCE | 2001年 / 4卷 / 07期

关键词：

D O I：

10.1038/89482

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Slit proteins are a family of secreted guidance proteins that can repel neuronal migration and axon growth via interaction with their cellular roundabout receptors (Robos). Here it was shown that Slit2-Robo-1 interactions were enhanced by cell-surface heparan sulfate. Removal of heparan sulfate decreased the affinity of Slit for Robe by about threefold. In addition, removal of cell-surface heparan sulfate by heparinase III abolished the chemorepulsive response to Slit2 normally shown by both the migrating neurons and growing axons. These results indicate essential roles for cell-surface heparan sulfate in the repulsive activities of Slit2.

引用

页码：695 / 701

页数：7

共 51 条

[31] COLLAPSIN - A PROTEIN IN BRAIN THAT INDUCES THE COLLAPSE AND PARALYSIS OF NEURONAL GROWTH CONES [J].

LUO, YL ;

RAIBLE, D ;

RAPER, JA .

CELL, 1993, 75 (02) :217-227

[32] Identification of high-molecular-weight proteins with multiple EGF-like motifs by motif-trap screening [J].

Nakayama, M ;

Nakajima, D ;

Nagase, T ;

Nomura, N ;

Seki, N ;

Ohara, O .

GENOMICS, 1998, 51 (01) :27-34

[33]

Niclou SP, 2000, J NEUROSCI, V20, P4962

[34] KINETICS OF BASIC FIBROBLAST GROWTH-FACTOR BINDING TO ITS RECEPTOR AND HEPARAN-SULFATE PROTEOGLYCAN - A MECHANISM FOR COOPERATIVITY [J].

NUGENT, MA ;

EDELMAN, ER .

BIOCHEMISTRY, 1992, 31 (37) :8876-8883

[35] CHEMOREPULSION OF AXONS IN THE DEVELOPING MAMMALIAN CENTRAL-NERVOUS-SYSTEM [J].

PINI, A .

SCIENCE, 1993, 261 (5117) :95-98

[36] Selecting a longitudinal pathway:: Robo receptors specify the lateral position of axons in the Drosophila CNS [J].

Rajagopalan, S ;

Vivancos, V ;

Nicolas, E ;

Dickson, BJ .

CELL, 2000, 103 (07) :1033-1045

[37] Crossing the midline: Roles and regulation of robo receptors [J].

Rajagopalan, S ;

Nicolas, E ;

Vivancos, V ;

Berger, J ;

Dickson, BJ .

NEURON, 2000, 28 (03) :767-777

[38] REQUIREMENT OF HEPARAN-SULFATE FOR BFGF-MEDIATED FIBROBLAST GROWTH AND MYOBLAST DIFFERENTIATION [J].

RAPRAEGER, AC ;

KRUFKA, A ;

OLWIN, BB .

SCIENCE, 1991, 252 (5013) :1705-1708

[39]

Ringstedt T, 2000, J NEUROSCI, V20, P4983

[40] SLIT - AN EXTRACELLULAR PROTEIN NECESSARY FOR DEVELOPMENT OF MIDLINE GLIA AND COMMISSURAL AXON PATHWAYS CONTAINS BOTH EGF AND LRR DOMAINS [J].

ROTHBERG, JM ;

JACOBS, JR ;

GOODMAN, CS ;

ARTAVANISTSAKONAS, S .

GENES & DEVELOPMENT, 1990, 4 (12A) :2169-2187

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