Purification and physicochemical properties of different polysaccharide fractions from the water extract of Boschniakia rossica and their effect on macrophages activation

被引：36

作者：

Liu, Yang ^{[1
]}

Sheng, Yu ^{[1
]}

Yuan, Guangxin ^{[2
]}

Wang, Yan ^{[1
]}

Wei, Huifang ^{[1
]}

Guan, Ming ^{[1
]}

Pei, Jin ^{[1
]}

机构：

[1] Jilin Univ, Sch Pharmaceut Sci, Changchun 130021, Peoples R China

[2] Beihua Univ, Pharmaceut Coll, Jilin 132013, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2011年 / 49卷 / 05期

关键词：

Boschniakia rossica; Polysaccharides; Purification; Physicochemical properties; Macrophages activation;

D O I：

10.1016/j.ijbiomac.2011.08.024

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Today more and more attentions had been attracted by many nutritionists and pharmacologists on polysaccharides from natural plants or animals due to their significant biological activities. In this research three polysaccharides (BRR-W1, BRR-WA1 and BRR-WA2) were isolated and purified from the water extract of Boschniakia rossica by DEAE Sepharose Fast Flow and Sepharose 6 Fast Flow column chromatography. chemical and physical characteristics of three polysaccharides were investigated by a combination of chemical and instrumental analysis methods. The assays of their effect on macrophages activation were also investigated in vitro, including phagocytosis of macrophages, detections for NO production and TNF-alpha secretion. The results indicated that the effect of polysaccharides on macrophages activation was influenced by their respective physicochemical properties. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：1007 / 1011

页数：5

共 17 条

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NEW METHOD FOR QUANTITATIVE-DETERMINATION OF URONIC ACIDS [J].